Energy drinks and oral non-keratinocytes (Histological & Immunohistochemical rat study) | ||||
| Ain Shams Dental Journal | ||||
| Volume 37, Issue 1, March 2025, Page 352-365 PDF (1.73 MB) | ||||
| Document Type: Original articles | ||||
| DOI: 10.21608/asdj.2024.315083.1473 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Fatma Adel Saad 1; Dina M Hassouna  2
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| 1Associate professor of Oral Biology- Faculty of Dental Medicine- King Salman International University-Egypt | ||||
| 2Lecturer of Oral Biology-Faculty of Dentistry- Fayoum University- Egypt | ||||
| Abstract | ||||
| Aim: To study the effect of the chronic consumption of Red-bull energy drink and its withdraw on oral non-keratinocytes in middle-aged adult albino rats. Materials and Methods: 24 adult male albino rats were divided into 3 groups (6 rats each). Group I (control) divided into Ia, Ib subgroups received 1.5ml/100g/day distilled water for 4 and 8 weeks respectively, group II (Red-bull group) received 1.5ml/100g/day RB orally for 4 weeks. Group III (withdrawal) received the above dose of RB for 4 weeks and was left for 4 weeks without any treatment. Paraffin sections of buccal mucosa were processed for H&E and immuno-histochemical examination of Melanocytes (HMB-45), Langerhans cells (CD1a) and Merkel cells (CK20). Results: Compared to control and withdrawal groups, H&E sections of Red-bull group displayed apparent increase of inflammatory and degenerative changes. H&E and immunohistochemical sections of group II revealed a significant increase in Langerhans cells, Merkel cells and melanocytes compared to group III and group I respectively. In group III, a significant decrease in Melanocytes, Langerhans cells and Merkel cells respectively was detected compared to group II. A significant increase in Langerhans’s cells was observed in all groups followed by Merkel cell then melanocytes. Conclusion: There was a significant increase in oral non-keratinocytes after chronic consumption of Red-bull drink, while a significant decrease was revealed the withdrawal group, despite the histopathological changes of the oral epithelium were not entirely restored to normal. Oral non-keratinocytes have a substantial role in immune and inflammatory reactions besides the renovation of oral epithelium. | ||||
| Keywords | ||||
| Redbull; HMB-45; CD1a; CK20; Oral non-keratinocytes | ||||
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