Toxicological study on Epothilone B which isolated from Aspergillus fumigatus | ||||
| Biochemistry Letters | ||||
| Volume 21, Issue 1, 2025, Page 16-28 PDF (833.39 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/blj.2025.314950.1064 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ashraf S.A. El-Sayed1; Ahmed Hussein Moustafa2; Noha. M. Saad  3; Mohamed Ali 4
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| 1Enzymology and Fungal Biotechnology lab, Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt. | ||||
| 2Chemistry Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt | ||||
| 3Biochemistry Department, Faculty of Dentistry, Sinai University, Kantara 41612, Egypt | ||||
| 4Chemistry department, Faculty of science, Zagazig university, Zagazig, Egypt | ||||
| Abstract | ||||
| Background: The myxobacterium Sorangium cellulosum produces epothilones, which are secondary metabolites that are macrolactones that have a broad anticancer effect on a variety of cancers with a higher propensity to stabilize their microtubule arrays during cellular division. Epothilones' higher water solubility contributes to their effective anticancer activity, which significantly affects tumor cells resistant to several drugs. Because of its water solubility, epothilone has a thousand-fold better effectiveness than taxol against tumors that are resistant to it, meaning that it can be used in vivo without the need for adjuvants. Aim: The aim of this study is designed to evaluate the anticancer activity of Epothilones as a natural products extracted from fungi and test it’s cytotoxic effect. Methods: extracted of epothilones from Aspergillus fumigatus were examined invitro on different cell lines And evaluate biochemical estimations of complete blood picture, liver function and kidney function were performed. Results: Invitro, IC50 of extracted epothilone B On different human lines liver cancer, colon cancer, prostate cancer, breast cancer and vero (normal cells) were found 6.32 ± 0.05 μM , 7.34 ± 0.21 μM , 7.6 ± 0.06 μM , 11.91 ± 0.24 μM and 18.77 ± 0.3 μM respectively. Conclusion: extracted of Epothilones has apotent antitumor activity on different cell lines, and as a drug may be safe compounds. | ||||
| Keywords | ||||
| epothilone B; anticancer activity; cytotoxicity; IC50; LD50 | ||||
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