Molecular detection of Toxoplasma gondii parasite in pregnant women in Diwaniyah Governorate | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 24 May 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.376862.2716 | ||
| Authors | ||
| Raheeq Faris Kadhim; Suhool Kareem Abdul Hassan; Rabab Hazim Elias; Ali Fadhil Abduljabbar* | ||
| Kut Technical Institute, Middle Technical University, Iraq | ||
| Abstract | ||
| Background: This study included detect Toxoplasma gondii infections among women through both serological and molecular diagnostic methods. A total of 182 serum samples were collected from pregnant women attending Al-Diwaniyah Teaching Hospital for Women and Children during 2022. Methods: Serological testing was performed to detect anti-T. gondii antibodies, and molecular detection was conducted using PCR targeting the B1 gene of the parasite. The relationship between infection prevalence and various factors, including the spouses of infected women (male partners) and place of residence, was also assessed. Results: The overall infection rate detected by conventional serological methods was 45.05%, whereas molecular detection yielded a lower prevalence rate of 27.22%. Additionally, 84 blood samples from the husbands of infected women were analysed, with infection rates of 36.90% by serology and 13.09% by PCR. Monthly distribution of infection rates revealed the highest prevalence in October (65.38%) and September (60%). Regarding geographical distribution, the infection rate was significantly higher among individuals residing in rural areas (67.07%) compared to those in urban settings (32.92%). Conclusion: These variations highlight the influence of demographic and environmental factors on the transmission dynamics and epidemiology of T. gondii within the studied population. | ||
| Keywords | ||
| Molecular; toxoplasma gondii parasite; infection | ||
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