Molecular detection and phylogenetic analysis of Canine Parvovirus (CPV) in diarrhetic k9 dogs in Thi-qar Province-Iraq | ||
| Microbial Biosystems | ||
| Article 13, Volume 10, Issue 2, June 2025, Pages 117-124 PDF (577.68 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mb.2025.342574.1215 | ||
| Author | ||
| Abbas Dakheel Mutar* | ||
| Animal Production Department, College of Agriculture and Marshes, University of Thi-Qar, Thi-Qar, Iraq. | ||
| Abstract | ||
| The present study was designed to the PCR diagnosis and DNA sequencing of canine parvovirus type 2 in diarrheal canine dogs. The results of 35 clinically suspected CPV-2 infected puppies showed diarrhea, vomiting (100%), dehydration (77%), and 25% of animals were dead. A genetic fragment of 400bp length that partially covered a coding portion of the CPV gene, within viral samples was amplified in this study. A direct sequencing strategy was performed for the observed PCR amplicons in the amplified genetic locus. Subsequently, a phylogenetic tree was constructed in the observed variants to assess the accurate phylogenetic distances alongside other relative viral sequences. The present results recorded that the presence of about 99% homology between studied samples with Canine parvovirus. Nine genetic variations of CPV gene were identified in Canine parvovirus; several genetic variation detected in all studied samples, while some variation were detected only in (S4,S5,S7) samples, like: A54G, A215G and T69C. | ||
| Keywords | ||
| Amplicon; Canine diarrhea; genetic fragment; nucleotide substitution; viral genome | ||
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