Impact of breast milk microbiota on the neonatal intestinal microbiota in the view of delivery type and circumstances, a cross-sectional study
Kamel, I., Ali, W., Mahmoud, W., Kamhawy, A., Armaneous, A., Hammad, D., Yousef, W., Basha, W., Mostafa, M., Allayeh, A., Sallam, M., El-Hariri, H., Mehanna, N., Ahmed, W. (2025). Impact of breast milk microbiota on the neonatal intestinal microbiota in the view of delivery type and circumstances, a cross-sectional study. EKB Journal Management System, (), -. doi: 10.21608/mid.2025.313591.2215
Iman H. Kamel; Walaa Hany Ali; Walaa Saad Mahmoud; Alyaa Hossny Kamhawy; Ayman Fekrey Armaneous; Doaa Yehia Hammad; Walaa Yousef; Walaa A. Basha; Mohammed Ibrahim Mostafa; Abdou Kamal Allayeh; Marwa Kamal Sallam; Hazem Mohamed El-Hariri; Nayra Shaker Mehanna; Wafaa Osman Ahmed. "Impact of breast milk microbiota on the neonatal intestinal microbiota in the view of delivery type and circumstances, a cross-sectional study". EKB Journal Management System, , , 2025, -. doi: 10.21608/mid.2025.313591.2215
Kamel, I., Ali, W., Mahmoud, W., Kamhawy, A., Armaneous, A., Hammad, D., Yousef, W., Basha, W., Mostafa, M., Allayeh, A., Sallam, M., El-Hariri, H., Mehanna, N., Ahmed, W. (2025). 'Impact of breast milk microbiota on the neonatal intestinal microbiota in the view of delivery type and circumstances, a cross-sectional study', EKB Journal Management System, (), pp. -. doi: 10.21608/mid.2025.313591.2215
Kamel, I., Ali, W., Mahmoud, W., Kamhawy, A., Armaneous, A., Hammad, D., Yousef, W., Basha, W., Mostafa, M., Allayeh, A., Sallam, M., El-Hariri, H., Mehanna, N., Ahmed, W. Impact of breast milk microbiota on the neonatal intestinal microbiota in the view of delivery type and circumstances, a cross-sectional study. EKB Journal Management System, 2025; (): -. doi: 10.21608/mid.2025.313591.2215

Impact of breast milk microbiota on the neonatal intestinal microbiota in the view of delivery type and circumstances, a cross-sectional study

Microbes and Infectious Diseases
Articles in Press, Accepted Manuscript, Available Online from 25 May 2025  XML
Document Type: Original Article
DOI: 10.21608/mid.2025.313591.2215
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Authors
Iman H. Kamel1; Walaa Hany Ali2; Walaa Saad Mahmoudorcid 3; Alyaa Hossny Kamhawyorcid 4; Ayman Fekrey Armaneous5; Doaa Yehia Hammad6; Walaa Youseforcid 7; Walaa A. Basha8; Mohammed Ibrahim Mostafa9; Abdou Kamal Allayeh10; Marwa Kamal Sallam email orcid 11; Hazem Mohamed El-Haririorcid 12; Nayra Shaker Mehanna13; Wafaa Osman Ahmed14
1Child Health Department, Institute of Medical Research and Clinical studies, National Research Center, Giza, Egypt
2Child Health Department, Institute of Medical Research and Clinical Studies, National Research Center, NRC, Cairo, Egypt
3Biological anthropology department, Institute of medical research and clinical studies, NRC , Egypt
4Child Health Department, Institute of Medical Research and Clinical studies, National Research Center, NRC, Cairo, Egypt
5Child Health Department, Institute of Medical Research and Clinical studies, National Research Center, NRC, Egypt.
6Biological Anthropology Department, Institute of medical research and clinical studies, NRC, Egypt
7Biological Anthropology Department, Institute of Medical Research and Clinical Studies, National Research Center, NRC, Cairo, Egypt
8Biological Anthropology Department, Institute of Medical Research and Clinical Studies, NRC, Egypt
9Clinical Pathology Department, Institute of Medical Research and Clinical Studies, National Research Center, NRC, Cairo, Egypt
10Virology lab 176, Water Pollution Research Department, Institute of Environment and Climate Change, National Research Centre, Dokki, Giza, Egypt
11Department of Medical Microbiology and Immunology, Faculty of medicine, Cairo University
12Department of Community Medicine, Institute of Medical Research and Clinical Studies, National Research Center, Cairo, Egypt
13Head of Probiotics Lab, Central Laboratories Network, Institute of Food Science and Nutrition, National Research Center, Dokki, Egypt.
14Pediatrics departnment, Faculty of Medicine , Ain Shams University, Cairo, Egypt
Abstract
Background: Bacterial colonization of the neonatal gut has imperative consequences on the health and development of the newborns. Aim: To assess the impacts of maternal breast milk microbiota and other perinatal factors on the colonization and construction of newborns’ gut microbiota. Methods: Specimens from mothers’ milk and neonatal stool, from 40 mother-infant pairs, were subjected to real time polymerase chain reaction (qPCR) analysis, to assess the effect of mothers’ milk microbiota on bacterial composition of the neonates’ gut in the view of different modes of delivery and different circumstances. Results: The study showed that Bifidobacterium was the most abundant bacterial species detected in both neonates’ stool and breast milk. There was a significant higher level of stool Bifidobacterium in neonates who were exclusively breastfed. Maternal milk Bifidobacterium increased significantly in mothers who breastfed their neonates exclusively. Antibiotics administration, either in neonates or their mothers, was negatively associated with the presence of stool Bifidobacterium and Streptococcus spp. Our results indicated that neonatal age, weight, sex, and mode of delivery showed no considerable association with any microbiota, either in the stool or milk. Milk Bifidobacterium and Streptococcus spp. decreased significantly in mothers receiving antibiotics; however, stool Staphylococcus spp. increased significantly in either neonates who needed respiratory support or their mothers lived in urban communities. Conclusion: Promotion of early and exclusive breast milk feeding for neonates has a great impact on shaping neonatal gut microbiota. Its importance becomes much augmented when neonates are admitted to NICUs and subjected to antibiotics, regardless of the mode of delivery.
Keywords
Breast milk; Neonatal stool; PCR; Vaginal delivery; Caesarean section
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