Impact of adding L-carnitine and semen preparation on human semen parameters after cryopreservation
Osman, A., Abu Elnaga, N., Abd –elmonem2, M., Hassan, E. (2025). Impact of adding L-carnitine and semen preparation on human semen parameters after cryopreservation. EKB Journal Management System, 4(1), 553-562. doi: 10.21608/ijtar.2025.350114.1105
Arwa Gamal Osman; Nehal Ali Abu Elnaga; Mohamed El-mahdy Abd –elmonem2; Eman Anwar Hassan. "Impact of adding L-carnitine and semen preparation on human semen parameters after cryopreservation". EKB Journal Management System, 4, 1, 2025, 553-562. doi: 10.21608/ijtar.2025.350114.1105
Osman, A., Abu Elnaga, N., Abd –elmonem2, M., Hassan, E. (2025). 'Impact of adding L-carnitine and semen preparation on human semen parameters after cryopreservation', EKB Journal Management System, 4(1), pp. 553-562. doi: 10.21608/ijtar.2025.350114.1105
Osman, A., Abu Elnaga, N., Abd –elmonem2, M., Hassan, E. Impact of adding L-carnitine and semen preparation on human semen parameters after cryopreservation. EKB Journal Management System, 2025; 4(1): 553-562. doi: 10.21608/ijtar.2025.350114.1105

Impact of adding L-carnitine and semen preparation on human semen parameters after cryopreservation

International Journal of Theoretical and Applied Research
Volume 4, Issue 1, June 2025, Page 553-562  XML PDF (461.45 K)
Document Type: Original Article
DOI: 10.21608/ijtar.2025.350114.1105
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Authors
Arwa Gamal Osman1; Nehal Ali Abu Elnaga email 1; Mohamed El-mahdy Abd –elmonem22; Eman Anwar Hassan3
1Embryology
2Obstetrics and Gynecology, Faculty of Medicine, Alexandria University, Alexandria, Egypt
3International Islamic Center for Population Studies and Research, Al-Azhar University, Cairo, Egypt.
Abstract
Abstract
Background: The use of antioxidants in semen extenders may reduce cryo-damage to sperm cells. This study assessed the impact of adding L-carnitine to the freezing medium for raw and prepared human semen after complete thawing. Methods: The normal semen samples were collected and divided into two main groups in raw and prepared labeled semen tubes (100 cases per group). Each group was under analysis before being divided into two subgroups: traditional cryoprotectant and L-carnitine addition sub-groups. The results showed no significant difference in sperm progressive motility rate between the L-Crantinin subgroup (C1: 11.01 ± 2.0%) and the control group (A1: 11.30 ± 1.2%). Moreover, in the cryoprotectant subgroup, the prepared semen showed a highly significant decrease (P ≤ 0.001) in mean DNA fragmentation compared to raw semen, with values of 18.01 ± 1.2% for prepared semen and 22.01 ± 1.2% for raw semen; similarly, in the subgroup that received cryoprotectant and L-carnitine. The prepared semen significantly decreased the average DNA fragmentation compared to raw semen (13.41 ± 2.5% versus 15.11 ± 2.2%, respectively). In conclusion, adding L-carnitine as an extender enhanced sperm motility, mitochondrial activity, and the integrity of the acrosomal and plasma membranes in frozen-thawed sperm to approach the non-cryopreserved state.
Keywords
semen; cryopreservation; L-carnitine
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