Evaluation of up-regulated miR-UL22A-3p gene expression in miscarriages women infected with Human cytomegalovirus as biomarker for pregnancy complications | ||
| Microbial Biosystems | ||
| Article 18, Volume 10, Issue 2, June 2025, Pages 160-166 PDF (524.4 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mb.2024.322165.1171 | ||
| Authors | ||
| Zaytoon Abdulrida Ighewish Al-Khafaji1; Müge Firat2; Ali Nadhim Shndiwe* 3 | ||
| 1Medicine College, University of Babylon, Babylon, Iraq. | ||
| 2Şabanözü Vocational High School, Veterinary Department, Çankırı Karatekin University, Çankırı, Turkey. | ||
| 3Iraq Ministry of Health, Babylon Health Directorate, Iraq. | ||
| Abstract | ||
| Human cytomegalovirus-miR-UL22A-3p (hcmv-miR-UL22A-3p) is a small, non-coding RNA molecule involved in the regulation of gene expression, playing a key role in various biological processes and diseases, including viral infections such as those caused by herpesviruses. This study aimed to evaluate the expression levels of hcmv-miR-UL22A-3p in women infected with Human cytomegalovirus (HCMV) and to assess its potential role in pregnancy outcomes, particularly miscarriage. A case-control study was conducted involving 50 women who experienced HCMV-related miscarriages and had a high viral load (>10×10³ copies/mL), alongside a control group of 50 healthy pregnant women with no history of miscarriage. Total RNA was extracted from buffy coat samples, and gene expression of hcmv-miR-UL22A-3p was quantified using Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR). Results demonstrated a significant upregulation of hcmv-miR-UL22A-3p in the miscarriage group, with a 2.92-fold change compared to the control group (1±0.0). Receiver Operating Characteristic (ROC) curve analysis revealed high diagnostic performance, with 92% sensitivity, 98% specificity, a confidence interval of 0.88–1.0, an area under the curve (AUC) of 0.91±0.3, and a cutoff value of 1.1 pg/mL (P = 0.000**). These findings suggest that elevated expression of hcmv-miR-UL22A-3p may be associated with HCMV-induced pregnancy loss and could serve as a potential biomarker for predicting miscarriage risk in HCMV-infected women. | ||
| Keywords | ||
| Biomarkers; gene expression; MicroRNAs; pregnancy loss; Real-Time PCR | ||
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