Molecular identification and detection of Opr1 and OprD virulence genes among Pseudomonas aeruginosa isolated from burn infections. | ||
| Biological and Biomedical Journal | ||
| Volume 3, Issue 2, July 2025, Pages 47-58 PDF (767.23 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bbj.2025.358224.1082 | ||
| Author | ||
| Dalia L. Hasan* | ||
| Department of Biology, Faculty of Science, University of Zakho, Kurdistan, Iraq | ||
| Abstract | ||
| Bacterial infection especially Pseudomonas aeruginosa is the leading cause of complications acquired from injuries that burn patients develop. The aim of the present study was to evaluate the rate of infection in burn wounds caused by Pseudomonas aeruginosa and to estimate the prevalence of some virulence genes. A total of 110 burn samples were collected from Burn and Plastic Surgery Hospital in Duhok City, Iraq. Pseudomonas aeruginosa isolates were identified using phenotypic and conventional biochemical tests and confirmed by the amplification of a species specific primer. The antibiotics susceptibility was determined by disk diffusion method and Polymerase chain reaction was used for the detection of the two virulence genes Opr1 and OprD. Out of the 110 tested samples from burn infection 71 were found to be positive for Pseudomonas aeruginosa with a rate of (64.54%). Females showed higher infection rates than males (59.15% vs 40.84%). The most effective antibiotic tested was found to be Colistin with a 100% rate while the highest level of resistant was detected with Piperacillin at a rate of (98.59%). The most common virulence gene encountered was OprD with a rate of (61.11%) followed by Opr1 at a rate of (57.4%). It is recommended that an antibiotic susceptibility test to be performed prior to therapy to improve the treatment options for patients. | ||
| Keywords | ||
| Pseudomonas aeruginosa; burn infection; antibiotic resistance | ||
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