Evaluation in vitro of the effect of bacteriophage as potential therapy for multidrug resistance uropathogenic Escherichia Coli | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 27 May 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.375175.2695 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ameera Tariq Ahmed  ; Thikra Ahmed Hamada
| ||||
| Department of Medical Microbiology, College of Medicine, University of Tikrite, Tikrite, Salahadeen, Iraq | ||||
| Abstract | ||||
| Background: Escherichia coli is the main cause of urinary tract infections (UTIs). Alternative therapeutic approaches are now required due to the drastically reduced treatment options caused by the rise of multidrug-resistant (MDR) uropathogenic E. coli (UPEC). Because of its efficacy and selectivity against bacteria, bacteriophage therapy has drawn interest as a possible remedy. The aim of the study was isolation and identification of MDR E.coli strains from UTI patients and investigation of the effect of bacteriophage as alternative therapy for these strains in vitro. Methods: A total of 75 urine samples were collected from the participants at 3 hospitals. Bacteriological and genetic methods are used for the isolation and identification of E.coli. Phenotyping and genotyping detection of MDR strain through antibiotic susceptibility test and PCR for CTX-M gene. Bacteriophages are isolated from the sewage of hospitals, and their effectiveness on MDR E. coli in vitro was evaluated and compared with traditional antibiotics. Result: 65 of the urine samples were culture positive. The E. coli was the predominant bacteria causing UTI 47(72.3%) among all isolates. The number of Uropathogenic Escherichia coli isolates that were MDR was 32 (68.08%). From 32 isolates of MDR, 17(53.12%) were positive for the CTX-M gene. The bacteriophage efficiency on E. coli shows sensitivity for MDR strains and causes lysis of bacteria on Muller Hinton media. Conclusion: The most frequent cause of nosocomial infections that are frequently linked to the emergence of MDR uropathogenic variations are E. coli isolates. Bacteriophage therapy has potential as a potent substitute for traditional antibiotics, especially when it comes to treating MDR UPEC. | ||||
| Keywords | ||||
| bacteriophages; MDR uropathogenic E.coli; urinary tract infection; alternative therapy | ||||
|
Statistics Article View: 66 |
||||
