Molecular study of Klebsiella pneumoniae isolated from different clinical and environmental sources in Mosul City | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 27 May 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.373198.2673 | ||
| Authors | ||
| Mohammed Abdul Razaq Al-Qatan* 1; Mohammed Zaghlool Saeed Al-Khayyat2 | ||
| 1Department of Biology, College of Science, University of Mosul, Mosul city, Iraq | ||
| 2Department of biology, College of Education for Pure Sciences, University of Mosul, Mosul city, Iraq | ||
| Abstract | ||
| Background: Klebsiella pneumoniae is considered one of the most dangerous and highly pathogenic bacteria due to its possession of many virulence factors, including antibiotic resistance genes. Therefore, this study aimed to identify some of its virulence factors, both phenotypically and molecularly, and thus the possibility of in silico inhibiting it using some natural fungal products. Methods: A total of twenty-nine isolates were collected and identified by biochemical and cultural tests, which were followed by 16S rRNA molecular diagnosis and sequencing to confirm the identification. The nucleotide sequence was submitted to the NCBI gene bank designated as PQ269274.1.A. Antibiotic sensitivity and plasmid profiling were also carried out for the selected isolate. For the identification of mucoid regulator rmpA, the qnr gene for quinolone resistance, and the blaCTX gene using specific primers, polymerase chain reaction (PCR) was used. Results: The results showed the presence of rmpA and qnr genes in all studied isolates, and blaCTX in only two isolates, which were isolated from urinary tract infections. Molecular docking was performed using beta-lactamase as a molecular target, using mushroom compounds, and all tested compounds had higher binding affinities than Penicillin G as a control. Conclusion: The presence of antibiotic resistance genes is important in disseminating resistance in the environment and risks to the antimicrobial therapy of infected patients. | ||
| Keywords | ||
| Quinolones resistance; PCR; mucoid regulator; Molecular docking | ||
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