Prevalence of Exo T toxin produced by Pseudomonas aeruginosa isolated from some hospitals in Iraq.

Ismaieel, Mohammed S.; Hassan, Muthanna Hamid; Flayyih, May T.

doi:10.21608/mid.2025.368369.2624

	Prevalence of Exo T toxin produced by Pseudomonas aeruginosa isolated from some hospitals in Iraq.
Microbes and Infectious Diseases
Articles in Press, Accepted Manuscript, Available Online from 27 May 2025
Document Type: Original Article
DOI: 10.21608/mid.2025.368369.2624
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Authors
Mohammed S. Ismaieel¹; Muthanna Hamid Hassan ¹; May T. Flayyih²
¹Department of biology, Collage of Science, University of Anbar, Iraq
²Department of Biology, College of Science, University of Baghdad, Iraq
Abstract
Background: ExoT is a type III secreted effector protein found in all P. aeruginosa strains and is essential for full virulence in severe pneumonia. Objective: This study aimed to determine the prevalence of exotoxin T among Pseudomonas aeruginosa isolates collected from hospitals in Ramadi City. Methods: A total of 71 isolates were isolation from various clinical an samples. The process of identifying the isolates was carried out utilizing standard procedures the ExoT was purified using ammonium sulphate, ion exchange and gel filtration chromatography (Sephadex G-150). Results: The prevalence of Pseudomonas aeruginosa in this study was 71 isolates out of 255 samples, representing 27.84%. These isolates showed high resistance rates of 100% to amoxicillin-clavulanic acid and Cephalothin, 67 isolates (94.37%) produced biofilms, intermediate producers showed the highest percentage (49.30%). 61 (86.91%) isolates harbored the Exotoxin T gene. isolate No. 23 was the highest Exotoxin T producer which was extracted and purified from, then was identified by PCR and ELISA. Conclusion: The bacteria in this study were characterized by their high resistance to both amoxicillin-clavulanic acid and Cephalothin antibiotics, in addition to their multiple ability to form biofilms and produce Exotoxin T at a high rate of 86.91%.
Keywords
Exotoxin T; P. aeruginosa; susceptibility; purification


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