Draft genome sequencing of Brachybacterium epidermidis strain TE.iq isolated from conjunctivitis of newborn in Mosul-Iraq | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 27 May 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.370186.2639 | ||||
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| Authors | ||||
Teeba Nashwan Salim   ; Essra Ghanim Alsammak
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| Department of Biology, College of Science, University of Mosul, Mosul, Iraq | ||||
| Abstract | ||||
| Background: The draft genome sequencing of Brachybacterium epidermidis provides insights into its genetic makeup and potential applications. This organism, belonging to the family Micrococcaceae, is notable for its role in various environments, including human skin microbiota. The sequencing efforts reveal critical genomic features that can be explored for biotechnological and medical applications. Aims: This study aimed to sequence the whole genome of an Iraqi Brachybacterium epidermidis isolate, designed as Brachybacteraium epidermidis srtain.TE.iq Methods: Swab samples are collected from newborns with conjunctivitis and cultured on various media. The Brachybacterium epidermidis isolates were confirmed using conventional PCR targeting the 16S rRNA gene. Specialized bioinformatics tools where it is used to assemble the genome, the annotation, the analysis of taxonomy based on an entire genome, in the hybridization of DNA and of an entire genome (isDDH), and detection of biosynthetic genes (BGC). Results: The genome sequence of Brachybacterium epidermidis strain TE.iq has been deposited at DDBJ/ENA/GenBank under the accession number JBKORE000000000. The results showed that Brachybacterium epidermidis strain TE.iq genome consist of 3,180,440 bp with GC content 70.47% and a total of 2,784 coding sequences, number of tRNA genes 49, number of rRNA genes 1 ,2,3 (5S ,16S ,23S) and N50 value of 215,932. Conclusion: The complete genome analysis of Brachybacterium epidermidis TE.iq is a significant step toward understanding the genetic characteristics of this bacterium, particularly it’s antibiotic resistance traits. The findings contribute to the broader knowledge of bacterial evolution, resistance mechanisms, and potential challenges in treating bacterial infections. | ||||
| Keywords | ||||
| Brachybacteraium epidermidis; whole genome sequencing; isDDH | ||||
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