Molecular Characterization and Targeting Vairimorpha ceranae Rad3-like Helicase through Docking Simulations for Novel Control Strategies... | ||||
| Bulletin of Faculty of Science, Zagazig University | ||||
| Article 11, Volume 2025, Issue 2, June 2025, Page 132-142 PDF (1.25 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfszu.2024.316254.1426 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Nahla A. M. Fathy   1; Ashraf S.A. Elsayed2; Hesham M. Abd El Halim3; Mai Labib4; Aly A. El-Sheikh1; Ahmed A. Bolbol5; Ahmed H. Moustafa6
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| 1Pest physiology department, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt | ||||
| 2Enzymology and Fungal Biotechnology Lab (EFBL), Botany and Microbiology Department, Faculty of Science, Zagazig University, 44519, Egypt | ||||
| 3Entomology Department, Faculty of Science, Benha University, Benha, Egypt. | ||||
| 4Agriculture Genetic Engineering Research Institute (AGERI), Agricultural Research Center, Giza, 12619, Egypt | ||||
| 5Botany and Microbiology Department, Faculty of Science, Zagazig University, 44519, Egypt | ||||
| 6Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt. | ||||
| Abstract | ||||
| Honeybee health, Apis mellifera, is threatened by microsporidian parasites like Vairimorpha ceranae and Vairimorpha apis, causing nosemosis. The study focuses on identifying Nosema species precisely and targeting V. ceranae's Rad3-like helicase for control methods in naturally infected apiary in Egypt. Microsporidian spores from adult worker bees were studied using microscopy, Polymerase Chain Reaction (PCR) with species specific primers, and sequencing analysis for comprehensive Nosema species identification. Accurate diagnosis plays a vital role in the implementation of focused control strategies. Subsequently, results revealed that all positive samples belonged to Nosema ceranae, indicating its presence in Egypt's Sharkia governorate. The study explores V. ceranae's Rad3-like ATP-dependent DNA binding helicase, vital for its DNA replication and repair processes. Inhibiting this enzyme could hinder parasite growth in bees. Computational simulations predict their effectiveness by identifying potential inhibitors for V. ceranae's Rad3-like helicase. This approach aids in finding drug candidates without extensive lab work. Integrating molecular characterization for Nosema sp. identification and analyzing V. ceranae's Rad3-like helicase as a drug target paves the way for precise control against nosemosis. In conclusion, Nosema ceranae presence in Egypt's Sharkia governorate is confirmed. Microscopy and PCR sequencing offer reliable identification of Nosema species. Targeting V. ceranae's Rad3-like ATP-dependent DNA binding helicase presents a promising path for future research and drug development against nosemosis. Further, in-silico studies are crucial to identify potential drug candidates with minimal off-target effects on honeybees. | ||||
| Keywords | ||||
| Nosema ceranae; Nosema apis; Apis mellifera; Rad3-like helicase; in-silico | ||||
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