Isolation and Identification of Proteus mirabilis from human and pets urine | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 31 May 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.386994.2813 | ||||
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| Authors | ||||
Hussein Ali Nasser  ; Basil A. Abbas; Ali B. Al-Deewan
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| College of Veterinary Medicine, University of Basrah, Basrah, Iraq | ||||
| Abstract | ||||
| Background: Proteus mirabilis is a Gram-negative bacterium known for its role in urinary tract infections (UTIs), particularly in catheter-associated UTIs (CAUTIs). It produces urease, which increases urine pH, leading to the formation of kidney and bladder stones. Methods: This study investigated the prevalence of P. mirabilis in urine samples collected from domestic pets (cats and dogs). A total of 150 urine samples (50 from human, 50 from cats and 50 from dogs) were obtained using catheterization and bladder massage. Samples were cultured on HiCrome UTI Agar, followed by identification using MacConkey agar and the VITEK system. Additionally, molecular identification was performed using PCR targeting the 16S rRNA gene. Results: Microscopic examination revealed an increase in red blood cells, white blood cells, and mucus, along with crystal formations. Culture results showed P. mirabilis infection in 26% of human (13/50), 4% of cats (2/50) and 0.0% in dogs. PCR analysis of 16SrRNA confirmed the presence of P. mirabilis in positive samples which different relations in phylogenetic analysis. Conclusion: The study results conclude that that the prevalence of Proteus mirabilis in urine samples was higher in cats compared to the dogs, but the highest rate was found in human samples. The isolated Proteus showed different phylogenetic relation. | ||||
| Keywords | ||||
| Proteus mirabilis; cat; dog; rRNA; crystal formations | ||||
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