Nematicidal efficacy of Spirulina platensis on egg hatching and second stage juveniles of Meloidogyne incognita in vitro

Allam, R. O. H.; Sweelam, M. E.; Mohanny, K. M.; Mohmed, Ghada S.; Ahmed, Rania A.

doi:10.21608/svuijas.2025.345709.1423

	Nematicidal efficacy of Spirulina platensis on egg hatching and second stage juveniles of Meloidogyne incognita in vitro
SVU-International Journal of Agricultural Sciences
Volume 7, Issue 2, June 2025, Page 1-9 PDF (666.17 K)
Document Type: Original Article
DOI: 10.21608/svuijas.2025.345709.1423
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Authors
R. O. H. Allam¹; M. E. Sweelam²; K. M. Mohanny¹; Ghada S. Mohmed¹; Rania A. Ahmed ¹
¹Plant Protection Department, Faculty of Agriculture, South Valley University, Qena, Egypt
²Economic Entomology and Agricultural Zoology Department, Faculty of Agriculture, Menoufia University, Shebin Elkom, Egypt
Abstract
This study was conducted at the Pesticides Laboratory of Plant Protection Department, Faculty of Agriculture, South Valley University, Qena, to assess the effect of the blue green alga, Spirulina platensis on the egg hatching process and second stage juveniles (J₂s) mortality of the root-knot nematode, Meloidogyne incognita at five different concentrations: 1000, 500, 250, 125 and 62.5 ppm in vitro. Effects were recorded after 2, 4 and 7 days of exposure for egg hatching, while J₂s mortality was determined after 24, 48, and 72 hours of application, compared to a control. The obtained results revealed that all tested concentrations of S. platensis inhibited egg hatching and caused high mortality of M. incognita J₂s compared to control. Regarding the toxic effects of S. platensis on M. incognita egg hatching, the maximum inhibition (99.45%) was recorded at a concentration of 1000 ppm, while the minimum inhibition (59.88%) was observed at 62.5 ppm. Meanwhile, the maximum J₂s mortality (93.65%) was observed at a concentration of 1000 ppm after 72 hours of application, with an LC₅₀ value of 0.23 ppm. The minimum mortality (43.81%) was recorded at a concentration of 62.5 ppm after 24 hours, with an LC₅₀ value of 0.69 ppm. It can be concluded that S. platensis has potential effect in controlling M. incognita and could be a possible replacement for chemical nematicides.
Keywords
Biocontrol; Meloidogyne incognita; Spirulina platensis; egg hatching; in vitro


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