Precipitin test to determining the relationship between some insect predators and fall armyworm and red spider mites
El Shamy, E., Abdel Hady, M., Abdelaziz, Y., Amer, N., Elsadawy, A., El-Shazly, S., Farag, A. (2025). Precipitin test to determining the relationship between some insect predators and fall armyworm and red spider mites. EKB Journal Management System, 3(1), 29-35. doi: 10.21608/jpfs.2025.356784.1024
Emad Abd El-ghafar El Shamy; Mohamed Abdel hady Hassan Abdel Hady; Yasmen A. Abdelaziz; Nahed S. Amer; Amany A. Elsadawy; Safaa A. El-Shazly; Abd El-Salam A. Farag. "Precipitin test to determining the relationship between some insect predators and fall armyworm and red spider mites". EKB Journal Management System, 3, 1, 2025, 29-35. doi: 10.21608/jpfs.2025.356784.1024
El Shamy, E., Abdel Hady, M., Abdelaziz, Y., Amer, N., Elsadawy, A., El-Shazly, S., Farag, A. (2025). 'Precipitin test to determining the relationship between some insect predators and fall armyworm and red spider mites', EKB Journal Management System, 3(1), pp. 29-35. doi: 10.21608/jpfs.2025.356784.1024
El Shamy, E., Abdel Hady, M., Abdelaziz, Y., Amer, N., Elsadawy, A., El-Shazly, S., Farag, A. Precipitin test to determining the relationship between some insect predators and fall armyworm and red spider mites. EKB Journal Management System, 2025; 3(1): 29-35. doi: 10.21608/jpfs.2025.356784.1024

Precipitin test to determining the relationship between some insect predators and fall armyworm and red spider mites

Journal of Plant and Food Sciences
Article 3, Volume 3, Issue 1, June 2025, Page 29-35  XML PDF (501.32 K)
Document Type: Original Article
DOI: 10.21608/jpfs.2025.356784.1024
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Authors
Emad Abd El-ghafar El Shamy1; Mohamed Abdel hady Hassan Abdel Hady email orcid 2; Yasmen A. Abdelazizorcid 3; Nahed S. Amerorcid 3; Amany A. Elsadawyorcid 3; Safaa A. El-Shazlyorcid 3; Abd El-Salam A. Farag4
1Department of Agriculture, Zoology and Nematology, Faculty of Agriculture, Al-Azhar University, Assiut, Egypt
2Dept of Agric. Zoology and Nematology. Fac. of Agric. Al-Azhar Univ. Egypt.
3Department of Agriculture, Zoology and Nematology, Faculty of Agriculture (Girls branch), Al-Azhar University, Cairo, Egypt
4Department of cotton pesticides evaluation, plant protection research institute, ARC, Giza, Egypt.
Abstract
This study was conducted to evaluate the relationship between the antiserum of the insect predator against the fall armyworm and some cotton pests. The double diffusion test in agar was used to determine which larvae predator, the green lacewing, Chrysoperla carnea, was feeding on the fall armyworm, Spodoptera frugiperda, the cotton mealy bug, Phenacoccus solenopsis, and the two-spotted spider mite, Tetranychus urticea, in the field. The precipitin technique was observed in agar gel for the insect pests. The reaction between the antiserum of Chrysoperla carnea against antigens S. frugiperda, P. solenopsis, and T. urticea showed positive reactions represented by 5, 6, and 3 precipitin lines, respectively. It devoured 48.65 spotted spider mite nymphs and 56.33 armyworm 1st instar larvae during 7.99 and 8.99 days, respectively. The negative reaction was recorded when antiserum of Chrysoperla carnea was reacted against the antigen of the other predator, Scymnus interruptus. Sharp reactions between the antiserum of the fall armyworm pest S. frugiperda and the predators' antigens, Coccinella undecimpunctata, Metasyrphus corolla, Cydonia veciniisis, and Paedrus alfierii, were represented by 5, 4, 2, and 1 precipitin lines, respectively. This method indicated variations between the insect pests and their predators, and all antisera gave positive serological reactions with homologous antigens and negative reactions with heterologous antigens.
Keywords
Precipitin; antiserum; antigens; insect predator
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