Determination of the inhibitory activity of the fungi extract Trichoderma koningii against some multidrug-resistant bacteria species | ||||
| Journal of Bioscience and Applied Research | ||||
| Article 1, Volume 11, Issue 2, June 2025, Page 346-357 PDF (430.15 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbaar.2025.431243 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Shaymaa Naji Dahham  1; Hayfaa Rajab Alwan2; Ahmed Hussein Dhayea3; Mohammed Nadhir Maaroof4
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| 1Department of Chemistry, College of Education for Pure. sciences/Tikrit university, Tikrit, Iraq | ||||
| 2Department of Biology, College of Education for pure sciences/Tikrit university. Tikrit, Iraq | ||||
| 3Salah Al-Din Education Directorate, Ministry of Education, Iraq | ||||
| 4Department of Biology, College of Sciences /Tikrit University, Tikrit, Iraq | ||||
| Abstract | ||||
| Twenty-two samples were collected from patients attending Tikrit Teaching Hospital, Tikrit, Iraq. The samples were cultured on mannitol salt agar, blood agar, and MacConkey agar. The results showed that 15 samples gave positive growth with 68.18%. Identification results showed 8 gram-positive bacteria isolates (53.3%) and 7 gram-negative bacteria isolates (46.7%). Staphylococcus aureus was the most isolated, were 4 isolates (26.66%), followed by Staph. epidermidis and E. coli were 3 isolates for each species (20%), and two isolates for each P. aeruginosa and K. pneumonia with 13.33% for each one, in addition, one isolate for each P. mirabilis and P. aeruginosa with 6.66% for each one. Sensitivity results showed that all isolates were resistant to most antibiotics used in the current study: Tobramycin, Cloxacillin, Metronidazole, Ceftazidime, and Carbenicillin. while some isolates were sensitive to Amikacin, Levofloxacin. E. coli is highly resistant to Meropenem, Ceftazidime, and Co-trimoxazole. Gram-positive isolates are highly resistant to Meropenem and ceftazidime. Aqueous extract of secondary metabolites of Trichoderma koningii showed a high inhibitory effect on Gram-positive bacteria, more than Gram-negative bacteria. P. aeruginosa showed high sensitivity with inhibition diameters of 20, 29, and 35 at concentrations of 50, 75, and 100%, respectively. While the alcoholic extract showed less inhibitory activity than the aqueous extract against isolates. E. coli was most sensitive to alcoholic extract, especially at concentrations of 57 and 100 %, with inhibition diameters of 25 and 29 mm, respectively. While Staph. aureus was highly sensitive to alcoholic extract with inhibition diameters 18,25,32 mm at concentrations 50,75, and 100%, respectively. Staph. haemolyticus. | ||||
| Keywords | ||||
| Trichoderma koningii; pathogenic bacteria; inhibition activity; Antibiotic resistance | ||||
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