Influence of Pseudomonas aeruginosa exotoxin A against breast cancer (MCF-7) cell line | ||||
| Microbial Biosystems | ||||
| Article 19, Volume 10, Issue 2, June 2025, Page 167-173 PDF (493.49 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mb.2025.357212.1245 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Najlaa Nabhan Yaseen   1; Muthanna Hamid Hassan2
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| 1Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. | ||||
| 2Department of Biology, College of Science, University of Anbar, Anbar, Iraq. | ||||
| Abstract | ||||
| Out of sixty-nine different clinical samples included UTI, otitis media, wound, and burn infections, fifty samples showed bacterial growth, while the remaining were negative for bacterial growth. Only thirty-one (62%) isolates were related to Pseudomonas aeruginosa. Results indicated that burn infections were the highest with this bacterial colonization. Detection of the exotoxin A was conducted by using an ELISA kit. Current results revealed that, out of thirty-one P. aeruginosa isolates, only twenty-one were able to produce exotoxin A. The isolate (P29) was selected based on its highest productivity of this toxin, reaching 29.24 ng/ml, in addition to partial purification steps for this toxin that had been conducted. The molecular weight of the exotoxin A had been determined and appeared as 65.33 kilodaltons after being compared with standard proteins. The highest concentration of exotoxin A was 400 µg/mL. The results showed that when exotoxin A, which was partially purified, was tested on the MCF-7 cell line for 72 hours at 37°C, it significantly stopped protein production by affecting elongation factor 2 through the action of ADP-ribosyl transferase, but it did not have a noticeable effect on normal human dermal fibroblasts-neonatal (HDFn). | ||||
| Keywords | ||||
| Clinical isolates characterization; exotoxin A quantification; burn wound microbiota; protein synthesis inhibition; Selective Cytotoxicity Assessment | ||||
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