CD49 in Optimization the Cologenicity of Mesenchymal Stem Cell Culture Passage_

Abo-El Wafa, Hasnaa Ahmed; Shawky, Samia Samir; Mahmoud, Ahmed Sedky; Taha, Ahmed Abdelrahem Ahmed; Ayed, Mamdouh Elsemary

doi:10.21608/egyfs.2025.431341

	CD49 in Optimization the Cologenicity of Mesenchymal Stem Cell Culture Passage_
The Egyptian Journal of Fertility and Sterility
Volume 29, Issue 3 - Serial Number 11106352, May 2025, Page 128-139 PDF (464.92 K)
Document Type: Original Article
DOI: 10.21608/egyfs.2025.431341
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Authors
Hasnaa Ahmed Abo-El Wafa¹; Samia Samir Shawky¹; Ahmed Sedky Mahmoud¹; Ahmed Abdelrahem Ahmed Taha ²; Mamdouh Elsemary Ayed²
¹Clinical Pathology Department, Faculty of Medicine, Sohag University, Sohag, Egypt
²Obstetrics and gynecology Department, Faculty of Medicine, Sohag University, Sohag, Egypt
Abstract
Background: Stem Cell Populations Expressing Integrin a6 (CD49f) expression has been founded in bone mar-row-derived mesenchymal stem cells (BM-MSCs), fe-tal urinary bladder obtained MSCs, and UCB-MSCs. It is viable that the expression of CD49f might affect the stemness of MSC culture. The goal of this work was to assess the best passage of MSC culture by CD 49 surface marker. Methods: This comparative study was performed on 15 umbilical cords (UC) collected at Gynecology and Ob-stetrics Department of Sohag University Hospitals, fresh samples was taken immediately after caesarean section, maternal age within 20-36 years old, newborn weight >1300:3500 gm. The UC is gathered and kept in a sterile specimen cup comprising 0.9% normal saline at 4C° till processing (for best result within 12 –24 h of birth). Results: CD49F and CD 73 were signiﬁcantly increased in Day 12 than Days 3,6, and 9. There was a signiﬁcant relation between CD73 and blood grouping at 6 days. There was no signiﬁcant relation between CD 49F and CD73 and fetal sex. There was a notable negative cor-relation between CD 49F and the time with maternal age at 9 days. There was no signiﬁcant correlation between CD49F and CD73 and time with GA. There was no signif-icant correlation between CD49F and time with last delivery. a signiﬁcant positive association existed between CD 73 and time with delivery at 6 days. Conclusion: Blood group and maternal age may inﬂu-ence MSC culture outcomes, with time-dependent varia-tions in surface marker expression.
Keywords
CD49; Optimization; Cologenicity; Mesenchymal Stem Cell Culture


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