Virulence Genes and Pathogenicity Assessment of Salmonella Isolated From Diseased Chickens | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 02 June 2025 PDF (900.71 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.356877.2635 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ali Amer   1; Ahmed Shabaan2; Nada Thabet3; Heba Badr 4
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| 1Animal Health Research Institute (AHRI) | ||||
| 2Reference laboratory for veterinary quality control on poultry production (RLQP), Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Dokki, Giza, Egypt. | ||||
| 3Animal production department, the Faculty of Agriculture, Beni Suef University, Beni Suef, Egypt. | ||||
| 4Head of the bacteriology unit -RLQP, Animal Health Research Institute, Dokki, Giza, Egypt. | ||||
| Abstract | ||||
| This study emphasizes Salmonella infections in broiler farms to monitor and notify the growing threat of antimicrobial resistance and its impact on public health. During the first half of 2024, 14% (14/100) of broiler farms were positive for Salmonella. Five serotypes were identified; the most prevalent were S. enteritidis (35%) and S. typhimurium (28%), followed by S. Kentucky (21%), and less commonly S. Infantis and S. virchow (7% each). Antibiotic susceptibility testing revealed multidrug resistance (MDR) across isolates, showing high resistance to lincomycin (85.7%) and significant resistance to β-lactams (Ampicillin: 42.8%, Ceftriaxone: 28.5%). Colistin sulfate demonstrated the highest efficacy with sensitivity percent 85.7%.The pathogenicity index (PI) evaluated for different serotyped found that there are four serotypes (S. enteritidis, S. typhimurium, S. kentucky, and S. virchow) which classified as intermediately pathogenic, while S. Infantis exhibited as low pathogenicity index. PCR analysis detected three virulence genes (invA, spvC, avrA) in all isolates, with percentage 100%. This study reinforces the critical function of virulence factors and MDR in Salmonella-associated pathogenicity and underscores the importance of robust biosecurity measures, prudent antibiotic use, and continued surveillance to mitigate risks in poultry production and public health. | ||||
| Keywords | ||||
| Salmonella infections; antimicrobial resistance; Salmonella serotypes; pathogenicity index | ||||
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