The Effect of Pine Bark Extract Loaded Nanoparticles on Diabetic Wound Healing: Histological and Immunohistochemical Studies | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Volume 56, Issue 13, December 2025, Page 413-422 PDF (1.58 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.375376.2786 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Aml HY Shaala   1; Ayman Atiba2; Mohammed Kassab3; Nemany Hanafy4; Foad Ahmed Farrag5; Mona Abdelftah Ali1
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| 1Histology and Cytology, veterinary medicine, Kafr Elsheikh, Kafr Elsheikh, Egypt | ||||
| 2Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafr Elsheikh University, Kafr Elsheikh, Egypt | ||||
| 3Department of cytology and histology, kafrelsheikh unversity, Egypt | ||||
| 4Nano medicine, Nanoscience and technology institute , Karleshiekh, Egypt | ||||
| 5Department of Anatomy, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt | ||||
| Abstract | ||||
| The problem of diabetic wounds has long been recognized as an unmet therapeutic challenge among medical society. In this study, we assess the wound healing potential of Pine Bark (PB) extract nanoparticles on diabetic wound healing through histopathological and immunohistochemical (IHC) studies. Eighteen Wister rats were divided into 3 groups (n = 6 each): control group (NC) (non-diabetic), diabetic control group (DC), and diabetic treated PB group (PN). Under general anesthesia, full-thickness wounds were conducted on the back of all the rats, and then the PB extract nanoparticles was applied topically to the wounds of the diabetic treated PB group. The percentage of wound areas was measured on days 0, 3, 6, 9, 12, 15, and 18 post-wounding. The wound granulation tissues were detected by histopathologic examination, and the CD31 micro vessels were investigated using immunohistochemistry. Our findings indicated that wounds treated with pine extract nanoparticles exhibited significantly accelerated closure rate compared to both the DC (p < 0.01) and the NC group (p < 0.05).In addition, the process of re-epithelialization was significantly improved in the PN compared to the DC (p < 0.0001) and the NC (p < 0.05). Angiogenesis was also increased in the PN wounds compared to the DC. These results suggest that Pine bark extract nanoparticles effectively promote both the rate and extent of diabetic wound healing. This study underscores the therapeutic potential of pine extract nanoparticles, as a natural product, as effective agents in enhancing wound healing in diabetic patients. | ||||
| Keywords | ||||
| Diabetic wounds; pine extract nanoparticle; chronic wound healing | ||||
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