Molecular detection of Salmonella typhi bacteria shedding from diarrheal patients with previous infection | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 03 June 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.378995.2738 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Marwa ghanim hashim   1; Eman Abid Fahad Alhasnawi2; Hind Makki Abdlwahid3; Yusur Fadhil Shallal4
| ||||
| 1Medical Microbiology Department, College of Medicine, Ibn Sina University of Medical and Pharmaceutical sciences, Baghdad, Iraq | ||||
| 2Medical Laboratories Techniques department, Institute of Medical Technologies/ Al-Mansour, Middle Technical University, Baghdad, Iraq | ||||
| 3Medical Laboratories Techniques Department, College of Health and Medical Techniques, University of Mashreq, Baghdad, Iraq | ||||
| 4Microbiology Department, College of Science, Al-Karkh University of Science, Baghdad, Iraq | ||||
| Abstract | ||||
| Background: Salmonella is the causative agent of salmonellosis. It is a rod-shaped gram-negative facultative anaerobe bacterium belonging to the Enterobacteriaceae family. Aim: To detect specific active genes of Salmonella typhi using molecular diagnosis following isolation and culture of the bacteria from stool samples on selective media. Methods: Across-sectional study had collection of (60) stool samples were collecting from Medical City Hospital, and clinics for internal medicine. Results: The stool culture is considered the gold standard test for the detection of Salmonella, the results of the (60) samples from patients with symptoms of diarrhoea and enteric fever showed that (18) patients from (60) samples were positive (30%) for Salmonella typhi bacteria, and the results of the rest isolates (42) were negative without growth proportion (70%). Conclusion: In this study we focused on more specific method screening. The result we reached was (18) case appear positive and other (42) case were negative, but when examined the same samples by PCR technology the ratio of positive result become higher than traditional ways of the bacteria. . | ||||
| Keywords | ||||
| Salmonella; shedding; diarrheal; PCR | ||||
|
Statistics Article View: 101 |
||||
