Cardioprotective Effects of Melatonin versus Myricetin on Arsenic-Induced Toxicity in Rats: Histological and Immuno-histochemical Study | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 04 June 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2025.386247.2275 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Rania Hamdy Elsyade 1; Heba Abdelrazak Abdelfatah 2; Eman A. El-Sawaf 3; amira ibrahim morsy  4
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| 1Anatomy and embryology, faculty of Medicine, Helwan university, Cairo, Egypt. | ||||
| 2Helwan university faculty of medicine | ||||
| 3Anatomy and embryology department, faculty of medicine, Helwan University | ||||
| 4anatomy department of school of medicine, Mansoura university, Mansoura, Egypt | ||||
| Abstract | ||||
| ABSTRACT: Background: Arsenic(As) based compounds were used as pesticides and herbicides. Arsenic intoxication represents a serious and widespread public health concern. It is associated with excessive generation of reactive oxygen species (ROS). Natural antioxidants, melatonin myricetin may offer protective effects against this cardiotoxicity. Aim of the study: Comparing the protective role of melatonin and myricetin on arsenic-induced myocardial damage in rats. Material and methods: Forty-eight adult male albino rats were segregated into four groups: Gp I (Control), Gp II (Arsenic), Gp III (Arsenic + Myricetin), and Gp IV (Arsenic + Melatonin). Blood samples were gathered for biochemical studies. The heart was dissected for histological and immunohistochemical studies and the results were statistically analyzed. Results: When Gp II (arsenic) was examined histologically, the heart showed shrinkage and cardiac muscle deformation, focal myocyte destruction, confirmed by an increase in expression of caspase-3. Specimens in Gp III (Arsenic + Myricetin) showed considerable decrease in number of mast cells, minor localised myocyte degeneration, and a moderate PAS reaction for glycogen. Branching muscle fibres with an acidophilic sarcoplasm and centrally positioned oval nuclei were seen in Gp IV (Arsenic + Melatonin); mast cells were reportedly sparse and had a positive PAS for glycogen granules. Specimens from the heart of Gp II (Arsenic) appeared with decreased cytoplasmic expression of BCL2. In Gp III (Arsenic + Myricetin), the expression was seemingly moderate in comparison to seemingly increased expression in Gp IV (Arsenic + Melatonin). Conclusion: This study demonstrates that melatonin amended the histological changes of the cardiac tissues intoxicated by arsenic more effectively than myricetin. This improvement is attributed to its antioxidant properties. Keywords: heart – arsenic – myricetin – Melatonin - antioxidant Running title: Protective effect of melatonin versus myricetin against arsenic-induced myocardial toxicity | ||||
| Keywords | ||||
| heart; arsenic; melatonin; myricetin; antioxidant | ||||
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