Genomic analysis of the wheat rhizosphere-isolated Streptomyces acrimycini Encodes Enzymes with Potential Applications in Biotechnology
Sallam, A., Tagyan, A., Mahmoud, E., Hozayen, W., Alkhalifah, D., Hozzein, W. (2025). Genomic analysis of the wheat rhizosphere-isolated Streptomyces acrimycini Encodes Enzymes with Potential Applications in Biotechnology. EKB Journal Management System, 34(1), 61-73. doi: 10.21608/cat.2025.380007.1377
Abdel-Aal A. Sallam; Aya I. Tagyan; Esraa Mahmoud; Walaa G. Hozayen; Dalal Hussien M. Alkhalifah; Wael N. Hozzein. "Genomic analysis of the wheat rhizosphere-isolated Streptomyces acrimycini Encodes Enzymes with Potential Applications in Biotechnology". EKB Journal Management System, 34, 1, 2025, 61-73. doi: 10.21608/cat.2025.380007.1377
Sallam, A., Tagyan, A., Mahmoud, E., Hozayen, W., Alkhalifah, D., Hozzein, W. (2025). 'Genomic analysis of the wheat rhizosphere-isolated Streptomyces acrimycini Encodes Enzymes with Potential Applications in Biotechnology', EKB Journal Management System, 34(1), pp. 61-73. doi: 10.21608/cat.2025.380007.1377
Sallam, A., Tagyan, A., Mahmoud, E., Hozayen, W., Alkhalifah, D., Hozzein, W. Genomic analysis of the wheat rhizosphere-isolated Streptomyces acrimycini Encodes Enzymes with Potential Applications in Biotechnology. EKB Journal Management System, 2025; 34(1): 61-73. doi: 10.21608/cat.2025.380007.1377

Genomic analysis of the wheat rhizosphere-isolated Streptomyces acrimycini Encodes Enzymes with Potential Applications in Biotechnology

Catrina: The International Journal of Environmental Sciences
Volume 34, Issue 1, June 2025, Page 61-73  XML PDF (1.73 MB)
Document Type: Original Article
DOI: 10.21608/cat.2025.380007.1377
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Authors
Abdel-Aal A. Sallam1; Aya I. Tagyan email orcid 2; Esraa Mahmoud3; Walaa G. Hozayen4; Dalal Hussien M. Alkhalifah5; Wael N. Hozzeinorcid 2
1Basic Science Department, Faculty of Oral and Dental Medicine, Nahda University, Beni-Suef, Egypt.
2Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
3Genetics Department, Faculty of Agriculture, Beni-Suef University, Beni-Suef, Egypt
4Biochemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
5Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia
Abstract
The growing demand for efficient microbial cellulase for lignocellulosic biomass degradation in bioenergy, food, and agricultural industries has driven exploration of actinomycetes from rhizosphere environments as novel enzyme sources. In this study, actinomycetes isolated from wheat rhizosphere soils led to the identification of Streptomyces acrimycini BUS-WH31, a strain demonstrating high cellulolytic activity and exceptional salt tolerance. Taxonomic characterization through 16S rRNA gene sequencing, morphological analysis, and chemotaxonomic profiling confirmed its taxonomic identity. Whole-genome sequencing revealed a linear chromosome of 4,936,986 base pairs with a GC content of 78.77%, enriched with genes involved in carbohydrate metabolism, antibiotic biosynthesis, and transport systems. Under optimized fermentation conditions, the strain produced thermostable cellulase enzymes with optimal activity at 60°C and pH 8.5, maintaining enhanced enzymatic activity in the presence of up to 8% NaCl. Additional enzymatic assays confirmed amylase, lipase, and protease production, underscoring its multifunctional biocatalytic potential. These findings position S. acrimycini BUS-WH31 as a robust candidate for industrial biomass conversion processes, particularly in saline environments, with genomic insights paving the way for targeted biotechnological applications in biofuel production.
Keywords
Cellulolytic enzymes; Carbohydrate-active enzymes (CAZymes); Industrial biotechnology; Streptomyces
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