Histological and Biochemical Comparative Study Between the Effect of Quercetin and Topiramate on Induced Diabetic Peripheral Neuropathy in Adult Male Albino Rats | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 10 June 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2025.377584.2256 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mai Amin Almoatasem 1; azza Saleh embaby2; salwa khaled mostafa  3; Asmaa Mahmoud Mostafa2; Fatma Elzahraa mohamed Abdellatef4
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| 1Histology and Cell Biology Department, Faculty of Medicine, Beni-Suef University, | ||||
| 2Histology department, Faculty of Medicine, Beni-Suef University | ||||
| 3faculty of medicine benisuef university | ||||
| 4Medical Histology & Cell Biology Department Faculty of Medicine-Beni-Suef University | ||||
| Abstract | ||||
| Abstract Introduction: Diabetic peripheral neuropathy (DPN) is a prevalent consequence for diabetes mellitus, significantly attributing to risk of amputation and mortality, if untreated. Aim of the Work: To emphasize the possible regenerative capacities of Quercetin and Topiramate against induced DPN in rat models. Material and Methods: 50 adult male albino rats were grouped as the follow; control group (Ia and Ib; n=10) and experimental group (II; n=40) injected once by alloxan (150mg /kg/i.p) then subdivided equally after 4 weeks from diabetic induction into 4 subgroups. Subgroup IIa remained without further treatment. Subgroup IIb received Quercetin (100mg/Kg), subgroup IIc treated with Topiramate (30mg/kg), and subgroup IId received Quercetin with Topiramate in the same doses of IIb & IIc respectively. All were given by gastric gavage for another 4 weeks then the blood samples were withdrawn for biochemical assessments. Sciatic nerves were harvested for histological analyses using H&E., Toluidine-blue, immunohistochemical staining for caspase-3 and S-100 immunoreactivity respectively. In addition to ultra-thin sections for electron microscopic examination followed by both morphometric and statistical studies. Results: Subgroup IIa emphasized altered biochemical and immunohistochemical features; significant decline in nerve fibres count with disturbed organization, abnormal myelin configurations, axonopathy and significant increase and decrease in caspase-3 and S-100 immunoreactivity. Subgroup IIb and subgroup IIc revealed comparable results to each other inform of partial improvements. Optimal improvements were clearly emphasized with combined therapy in subgroup IId. Conclusion: Concomitant administration of Quercetin and Topiramate proved to exert better regenerative results than each treatment alone in experimentally induced diabetic neuropathy | ||||
| Keywords | ||||
| diabetic neuropathy; topiramate; quercetin | ||||
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