Evaluation of Physiochemical Elicitors on Production of Saponins and Alkaloids From Cestrum Diurnum Callus, and Assessment Of The Potential in Vivo Wound Healing Activity of Leaf Extract.

Alrabayah, Ibrahim N.; Elhawary, Seham S.; Kandil, Zeinab A.; Abd El-Kadder, Essam M.; Moemen, Yasmine S.; Saleh, Abdulrahman M.; Abd Elhamied, Nebal S.; El Raey, Mohamed A.

doi:10.21608/hrj.2025.432788

	Evaluation of Physiochemical Elicitors on Production of Saponins and Alkaloids From Cestrum Diurnum Callus, and Assessment Of The Potential in Vivo Wound Healing Activity of Leaf Extract.
Horticulture Research Journal
Volume 3, Issue 3, June 2025, Page 52-70 PDF (2.63 MB)
Document Type: Original Article
DOI: 10.21608/hrj.2025.432788
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Authors
Ibrahim N. Alrabayah¹; Seham S. Elhawary¹; Zeinab A. Kandil¹; Essam M. Abd El-Kadder²; Yasmine S. Moemen³; Abdulrahman M. Saleh⁴; Nebal S. Abd Elhamied²; Mohamed A. El Raey⁵
¹Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Giza , Egypt
²Trees Research Department, Horticulture Research Institute, Agriculture Research Center, Giza , Egypt
³Clinical Pathology Department, National Liver Institute, Menoufia University, Menoufia, Egypt
⁴Pharmaceutical Medicinal Chemistry & Drug Design Department, Faculty of Pharmacy (Boys),Al-Azhar University, Cairo, Egypt
⁵Phytochemistry and Plant Systematics, National Research Centre, Dokki, Cairo , Egypt
Abstract
Cestrum diurnum L. is a plant of significant medicinal interest, but traditional cultivation methods are time-consuming and uncontrolled. Callus culture offers a more efficient and reliable alternative for consistent secondary metabolite production. Among 19 combinations of plant growth regulators, MS medium supplemented with 2.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.25 mg/L thidiazuron (TDZ) yielded the highest callus formation. The impact of abiotic elicitors (UV radiation and polyethylene glycol) on callus growth and secondary metabolite production were studied. Results showed that addition of 50 mg/L tryptophan + UV and 25 mg/L tryptophan + UV resulted in the best biomass production, with fresh weights of 3.75±0.35 and 3.20±0.29 g/jar and dry weights of 0.452±0.01 and 0.407±0.04 g/jar, respectively. The highest saponin content was observed with 100 mg/L cholesterol, while alkaloid content increased with 50 mg/L tryptophan and 1 g/L PEG. Chronic wounds, burns, and infections remain major clinical challenges. Therefore, it was necessary to consider an alternative or adjuvant medicine that has low side effects and is safe at the same time as a natural source of medicinal plants. Leaves of C. diurnum were extracted using n-hexane and 70% ethanol, and both extracts were formulated into gels for testing. The n-hexane-200 group showed the highest healing rate, although differences among treated groups were not statistically significant. This study highlights the dual potential of C. diurnum in biotechnological metabolite production and therapeutic wound care.
Keywords
Cestrum diurnum L; Tissue culture; In; vivo; Wound healing; Plant growth regulators


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