Oral Supplementation of Alginate and Spirulina as Immunostimulant of Non-Specific Immune System of Litopenaeus vannamei | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 148, Volume 29, Issue 3, May and June 2025, Page 2653-2670 PDF (371.82 K) | ||||
| DOI: 10.21608/ejabf.2025.433791 | ||||
 View on SCiNiTO | ||||
| Abstract | ||||
| Litopenaeus vannamei gains a high export demand value. However, the productivity has decreased in the recent years due to several problems, such as infectious diseases. This study aimed to determine the effect of alginate and spirulina extract diet supplementation as an immunostimulant agent in increasing the non-specific immunity of L. vannamei. The study was conducted with four treatments, namely control, the addition of 3g of alginate in 1.0kg of feed (alginate 3g/kg), 5mg spirulina in 1.0kg of feed (spirulina 5 mg/kg), and 3g of alginate + 5 mg spirulina in 1kg of feed (alginate 3g/kg+spirulina 5mg/kg). The shrimps were reared for 12 days, and the cellular immune parameters were observed on days 0, 4, 8, and 12. The parameters determined in this study were the THC (total hemocyte count), PA (phagocytic activity )/PI (phagocytic index), the activity of PO (phenoloxidase), and SOD (superoxide dismutase). Generally, the results showed that the supplementation treatments were significantly different from the control (P<0.05). Highest THC value was found in the alginate 3g/kg+spirulina 5mg/kg treatment on day 12, the PA value in the alginate 3g/kg + spirulina 5mg/kg treatment on day 12, while an increase in PI was shown in the alginate 3g/kg+spirulina 5mg/kg treatment on day 12. The highest PO activity was shown in the treatment of alginate 3g/kg+spirulina 5mg/kg on day 4, while the highest activity of SOD was shown by treatment spirulina 5mg/kg on day 8. It is concluded that supplementation with alginate and spirulina extract enhances all parameters of the non-specific immune system in Litopenaeus vannamei. | ||||
| Keywords | ||||
| Alginate; Immune; Shrimp; Spirulina | ||||
|
Statistics Article View: 49 PDF Download: 61 |
||||
