Some Studies on Mycoplasma of Ostrich | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 19 June 2025 PDF (1.25 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.361349.2657 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Doaa Sobhy Abdel Aziz  1; Magda M.A. Mostafa1; Amal H.T. Abdelanaser1; Sabry Ismail Eissa2; Sahar El-Sayed Ouda Ahmed 2
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| 1Department of Poultry Diseases, Faculty of Veterinary Medicine, Benha University, Benha, Egypt | ||||
| 2Mycoplasma Department, Animal Health Research Institute, ARC. Giza, 12618, Egypt | ||||
| Abstract | ||||
| Mycoplasmas in ostriches are the primary causative agents of respiratory diseases, resulting in significant stock losses, reduced production and hatchability, and downgrading of carcasses, leading to substantial economic losses in the industry. The present study is considered the first record for the isolation of three Mycoplasma isolates from ostriches experiencing respiratory signs, arthritis, and conjunctivitis with identification accomplished through PCR using common Mycoplasma primers and 16SrRNAgene for sequencing. Ostriches between 5-6 months of age showed the highest prevalence of isolates with respiratory signs, arthritis, and conjunctivitis (90%), while those aged 12 months showed 60% prevalence. 90 tracheal and ocular dry swabs were examined for the presence of Mycoplasma infection, from an ostrich farm in AL Obour city in Egypt, where sheep were also being raised nearby. The extracted DNA from each swab were pooled in 18 groups, each consists of five pooled DNA samples. These groups were negative for Mycoplasma gallisepticum and Mycoplasma synoviae by PCR. The three sequenced 16SrRNAgenes represent strains from male, female and young age ostriches. They were submitted to GenBank under accession no. OR511890, OR511891 with96% similarity to M.struthionis, and M. falcons respectively. While, the isolate (OR 511889) showed high nucleotide identity (99%) for M. arginini. In conclusion, three new Mycoplasmas were isolated from Ostrich farm with respiratory manifestation for the first time in Egypt. Which is a foundation, for further studies on a large number of ostrich farms for a wide scale investigation and control programs. | ||||
| Keywords | ||||
| M. struthionis; M.Arginini; M. Falconis; PCR; sequencing | ||||
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