Phylogeny of Three Species Belonging to Genus Potyvirus Infecting Solanum tuberosum Based on Biological and Molecular Variability
El-Turkey, A., El-Attar, A., Othman, B., El Dougdoug, K. (2025). Phylogeny of Three Species Belonging to Genus Potyvirus Infecting Solanum tuberosum Based on Biological and Molecular Variability. EKB Journal Management System, 33(1), 21-34. doi: 10.21608/ajs.2025.319743.1583
Aya Hussin El-Turkey; Ahmed Kamal El-Attar; Badawi Abdel Salam Othman; K. A. El Dougdoug. "Phylogeny of Three Species Belonging to Genus Potyvirus Infecting Solanum tuberosum Based on Biological and Molecular Variability". EKB Journal Management System, 33, 1, 2025, 21-34. doi: 10.21608/ajs.2025.319743.1583
El-Turkey, A., El-Attar, A., Othman, B., El Dougdoug, K. (2025). 'Phylogeny of Three Species Belonging to Genus Potyvirus Infecting Solanum tuberosum Based on Biological and Molecular Variability', EKB Journal Management System, 33(1), pp. 21-34. doi: 10.21608/ajs.2025.319743.1583
El-Turkey, A., El-Attar, A., Othman, B., El Dougdoug, K. Phylogeny of Three Species Belonging to Genus Potyvirus Infecting Solanum tuberosum Based on Biological and Molecular Variability. EKB Journal Management System, 2025; 33(1): 21-34. doi: 10.21608/ajs.2025.319743.1583

Phylogeny of Three Species Belonging to Genus Potyvirus Infecting Solanum tuberosum Based on Biological and Molecular Variability

Arab Universities Journal of Agricultural Sciences
Volume 33, Issue 1, June 2025, Pages 21-34    PDF (711.83 K)
Document Type: Original Article
DOI: 10.21608/ajs.2025.319743.1583
Authors
Aya Hussin El-Turkey* 1; Ahmed Kamal El-Attar1; Badawi Abdel Salam Othman2; K. A. El Dougdoug2
1Virus Research Dept, Plant Pathology Research Institute, ARC, Giza 12619, Egypt
2Agricultural Microbiology Dept, Fac of Agric, Ain Shams Univ, P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt
Abstract
Potato virus Y exhibits diverse strains and complexities circulat-ing within potato fields, leading to yield deterioration. This study examined potato virus Y (PVY) isolates for their varied symptom expressions on po-tato plants. The infected potato plants were categorized into three distinct phenotypic expressions: venial necrosis (PVY-VN), yellow mosaic (PVY-YM), and mottling pattern (PVY-MP). They showed various biological in-dicators, including disease density index, pathogenicity, virus concentration via DAS-ELISA, and molecular variability through HC-PRO silencing gene accumulation via qRT-PCR. The phylogeny of the HC-PRO silencing gene revealed variations in similarity and genetic distance, corresponding to dif-ferences in the 207 nucleotides. These variations were observed among the three PVY isolates and between them and other PVY strains registered in the GenBank database. They were registered in GenBank under the acces-sion numbers #OR804503, #PQ015611, and #PQ015612, corresponding to PVY-VN, PVY-YM, and PVY-MP, respectively. Based on the phylogeny, the three isolates belong to the NTN strain and exhibit a sensitivity range of 99% to 100% with AJ585342_United-Kingdom-strain_NTN_sequence and KY092173_Poland_strain_sequence. 
Keywords
Phylogeny; PVY; DAS-ELISA; RT-PCR; qRT-PCR; Potato
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