Isolation and molecular identification of entomopathogens microorganisms infecting mulberry silkworm, Bombyx mori L. in Egypt
Mahmoud, K., Fouad Ewies, H., Mohamed ismail, I. (2024). Isolation and molecular identification of entomopathogens microorganisms infecting mulberry silkworm, Bombyx mori L. in Egypt. EKB Journal Management System, 13(1), 45-51. doi: 10.21608/japp.2025.383746.1021
Kariman Mahmoud; Hala Fouad Ewies; Ismail Mohamed ismail. "Isolation and molecular identification of entomopathogens microorganisms infecting mulberry silkworm, Bombyx mori L. in Egypt". EKB Journal Management System, 13, 1, 2024, 45-51. doi: 10.21608/japp.2025.383746.1021
Mahmoud, K., Fouad Ewies, H., Mohamed ismail, I. (2024). 'Isolation and molecular identification of entomopathogens microorganisms infecting mulberry silkworm, Bombyx mori L. in Egypt', EKB Journal Management System, 13(1), pp. 45-51. doi: 10.21608/japp.2025.383746.1021
Mahmoud, K., Fouad Ewies, H., Mohamed ismail, I. Isolation and molecular identification of entomopathogens microorganisms infecting mulberry silkworm, Bombyx mori L. in Egypt. EKB Journal Management System, 2024; 13(1): 45-51. doi: 10.21608/japp.2025.383746.1021

Isolation and molecular identification of entomopathogens microorganisms infecting mulberry silkworm, Bombyx mori L. in Egypt

Journal of Applied Plant Protection
Volume 13, Issue 1, 2024, Page 45-51  XML PDF (2.98 MB)
Document Type: Original Article
DOI: 10.21608/japp.2025.383746.1021
View on SCiNiTO View on SCiNiTO
Authors
Kariman Mahmoud email 1; Hala Fouad Ewies2; Ismail Mohamed ismail3
1Dept. of Plant Protection, Faculty of Agriculture, Suez Canal University, ISMAILIA 41522, EGYPT
22 Plant Protection Research Institute (PPRI), Agricultural Research Center (ARC), Dokki, Giza, Egypt
33 Department of Microbial Molecular Biology, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza 12619, Egypt.
Abstract
Sericulture, silkworm rearing, is an economically important agro-industry, particularly in developing countries. However, silkworm production is frequently hampered by various diseases caused by entomopathogens, leading to significant economic losses. Accurate and rapid identification of these pathogens are crucial for effective disease management strategies. Current study delves into the application of various molecular techniques, including PCR-based methods, sequencing, and phylogenetic tree using MEGA 11 program, to identify bacteria and fungi isolated from infected mulberry silkworm larvae. Results showed that two fungi and one bacterium species were isolated, identified and submitted to GenBank to have an accession number. Sequence alignments of the fungal isolates SHK-F1 and SHK-F2 showed identities 100 % with isolate Aspergillus flavus AJ38 and Penicillium oxalicum, respectively. However, the 16S rDNA gene amplicon obtained from bacterial isolate strain (SKH-B1) showed identity 99 %to the partial sequence of the gene in Bacillus velezensis. This is the first record of Penicillium oxalicum and Bacillus velezensis as entomompathogenic form B. mori L.
Keywords
Sericulture - Molecular biology; A. flavus; P. oxalicum; B. velezensis
Statistics
Article View: 58
PDF Download: 34