Role of TLR9 Gene Polymorphism among Patients with Exacerbation COPD | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Articles in Press, Accepted Manuscript, Available Online from 21 June 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2025.373812.2514 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Maha Mohammed Gamal  1; Heba Atia Yassa2; Michael Nazmy agban3; Hoda Ahmed Makhlouf4
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| 1molecular microbiology, Molecular Biology Research Institute , Assiut University | ||||
| 2Professor of Forensic medicine and clinical toxicology, Faculty of medicine, and Vice dean of Molecular Biology research Institute, Assiut University, Egypt | ||||
| 3Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt, | ||||
| 4Department of Chest Diseases,Vice Dean for Community Services and Environmental Development, Faculty of medicine, Assiut University | ||||
| Abstract | ||||
| Toll-like receptor (TLR)-9 plays an important role in innate immunity by recognizing bacterial CpG-DNA motifs. It is not clear whether single nucleotide polymorphisms (SNPs) in TLR-9 have a role in the severity or susceptibility of developing ECOPD. Twenty-nine patients with ECOPD were selected according to the inclusion criteria, then examined for dyspnea scale, respiratory function test, and blood gases, after that 3ml of blood were taken to examine SNP in the promoter region of the TLR-9 gene (T1237C) and comparing to 29 healthy smokers as a control group. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis were used for genotyping.There were two genotypes for SNP of TLR-9 GENE among ECOPD patients; TC and TT with frequency of 24 (82.7%) and 5 (17%) respectively, whereas there were two genotypes among control TC and TT with frequency of 12 (41.4%) and 17 (58.6%).There is a significant role of genetic polymorphisms, particularly the T1237C SNP, in modulating susceptibility to ECOPD. | ||||
| Keywords | ||||
| COPD; ECOPD; Gene polymorphism; TLR9SNP; Smoking | ||||
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