Luteolin mitigates deoxycorticosterone acetate-salt-induced hypertension in rats by activation of Nrf2/HO-1 pathway

Elmorshdy, Shorouk E. M.; Khodir, Suzan A; Abd El-aziz, Noha M.; Abu-zeid, Aya Yousri; Megahed, Mohamed Osama; Ebeid, Mai A.; Abouelenin, Mai A. H.; Zaher, Shaimaa Mohammed; Abdelhameed, Mohamed A.; Hussein, Samar M.; Mansour, Rania Salah

doi:10.21608/muj.2025.386368.1226

	Luteolin mitigates deoxycorticosterone acetate-salt-induced hypertension in rats by activation of Nrf2/HO-1 pathway
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Articles in Press, Accepted Manuscript, Available Online from 23 June 2025 PDF (799.01 K)
Document Type: Original Article
DOI: 10.21608/muj.2025.386368.1226
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Authors
Shorouk E. M. Elmorshdy¹; Suzan A Khodir ²; Noha M. Abd El-aziz³; Aya Yousri Abu-zeid⁴; Mohamed Osama Megahed⁵; Mai A. Ebeid⁶; Mai A. H. Abouelenin⁷; Shaimaa Mohammed Zaher⁸; Mohamed A. Abdelhameed⁹; Samar M. Hussein¹⁰; Rania Salah Mansour¹¹
¹Medical Physiology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
²Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
³Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
⁴Pathology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
⁵Internal Medicine Department, Nephrology Unit, Faculty of Medicine, Damietta University, Egypt
⁶Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Egypt
⁷Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Menoufia, Egypt
⁸Histology and Cytology Department, Faculty of Medicine, Helwan University, Egypt
⁹Cardiology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
¹⁰Medical Physiology Department, Faculty of Medicine, Suez Canal University, Egypt
¹¹Medical Physiology Department, Faculty of Medicine, Ain Shams University, Egypt.
Abstract
Background: One of the risk factors for vascular disorders is hypertension. Several characteristics of human hypertension are replicated in the deoxycorticosterone acetate (DOCA) salt rat model. A common flavonoid having anti-oxidant and anti-inflammatory impacts is luteolin (lut). Aim of the study: to illustrate Lut's vascular protective effect and its underlying mechanisms in DOCA-induced hypertension. Material and methods: Thirty male albino rats were split into three: DOCA, DOCA+Lut, and control (10/group). Rats underwent ABP evaluation in addition to measurements of aortic gene expression of Nrf2 and HO-1, serum cholesterol, serum triglycerides, aortic MDA, aortic SOD, aortic TNF-α, aortic IL-6, and aortic IL-10. Aortic immunohistochemical and histological studies were also carried out. Results: Aortic SOD and aortic gene expression of Nrf2 and HO-1 values were dramatically decreased in the DOCA than in the control, but the measured SBP, DBP, MABP, serum levels of cholesterol and triglycerides, aortic MDA, aortic TNF-α, and aortic IL-6 were all dramatically increased in the DOCA than in the control. In addition, DOCA's immunoreactivity to Caspase-3 and aortic NF-kB was higher than that of the control. Lut significantly reduced the vascular alterations brought on by DOCA. Conclusion: By upregulating the Nrf2/HO-1 signaling pathway and having anti-inflammatory, anti-apoptotic, and antioxidant effects, luteoin has a vascular-protective effect in DOCA-induced hypertension.
Keywords
DOCA HO-1; Luteolin NF-kB; Nrf2 Vasculopathy


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