Albiflorin mitigates diabetic nephropathy in rats by down-regulation of TLR4/NF-kB signaling pathway

Hussein, Samar M.; Khodir, Suzan A; Amer, Ghada S; Abd El-aziz, Noha; Abu-zeid, Aya Yousri; Elkholy, Aly Mohamed; Mohammed, Maha Tarek; Abouelenin, Mai A. H.; Abdelfatah, Heba Abdelrazak; Elnahrawy, Esraa A

doi:10.21608/muj.2025.391322.1229

	Albiflorin mitigates diabetic nephropathy in rats by down-regulation of TLR4/NF-kB signaling pathway
Medicine Updates
Articles in Press, Accepted Manuscript, Available Online from 24 June 2025 PDF (1.18 MB)
Document Type: Original Article
DOI: 10.21608/muj.2025.391322.1229
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Authors
Samar M. Hussein¹; Suzan A Khodir ²; Ghada S Amer³; Noha Abd El-aziz⁴; Aya Yousri Abu-zeid⁵; Aly Mohamed Elkholy⁶; Maha Tarek Mohammed⁷; Mai A. H. Abouelenin⁸; Heba Abdelrazak Abdelfatah⁹; Esraa A Elnahrawy¹⁰
¹Medical Physiology Department, Faculty of Medicine, Suez Canal University, Egypt
²Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
³Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
⁴Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
⁵Pathology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
⁶Internal Medicine Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
⁷Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Egypt
⁸Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Menoufia, Egypt
⁹Histology Department, Faculty of Medicine, Helwan University Egypt
¹⁰Physiology Department, Badr University in Cairo (BUC), Cairo, Egypt.
Abstract
Introduction: Diabetic nephropathy (DN) is severe medical condition. It has been shown that albiflorin (ALB) has potent pharmacological effects in preventing oxidation, inflammation, and apoptosis. Objective: to evaluate the possible underlying mechanisms and the protective effect of ALB in STZ-induced DN. Materials and Methods: There were three groups of thirty adult male albino rats: control, DN, and DN+ALB (10/group). blood levels of glucose, glycosylated Hb A1c, cholesterol, triglycerides, urea, creatinine, renal SOD, creatinine clearance, renal MDA, renal TNF-α, renal IL-6, and renal genes expression of TLR4 and NF-kB. Additionally, immunohistochemical and renal histopathology analyses were carried out. Results: Serum levels of glucose, glycosylated Hb A1c, cholesterol, triglycerides, urea, and creatinine were significantly elevated in conjunction with urine albumin, renal MDA, renal TNF-α, renal IL-6, and renal genes TLR4 and NF-kB expression of DN in comparison to the control. It also significantly decreased creatinine clearance values and renal SOD expression. Furthermore, compared to the control, the DN group's TLR4 and NF-kB immunoreactions were both increased. The STZ-induced DN alterations significantly improved in the DN+ALB group. Conclusion: ALB offers substantial protection against STZ-induced DN through hypoglycemic, anti-oxidant and anti-inflammatory mechanisms as well as the down-regulation of TLR4/NF-kB signaling pathway.
Keywords
Albiflorin; Diabetic Nephropathy; NF-kB; TLR4


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