Growth Kinetics of the Rat Mesenchymal Stem Cells from the Bone Marrow and Adipose Tissue. A Comparative Study | ||
| The Egyptian Journal of Anatomy | ||
| Article 1, Volume 41, Issue 1, January 2018, Pages 1-9 PDF (766.87 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejana.2018.43497 | ||
| Authors | ||
| Mohamed El-Badry Mohamed1; Mona H. Mohamed Ali2; Maha Mohamed Abo-gazia3; Rania Abdel-Azim Galhom2; Amira El-Sayed farage4 | ||
| 1Department of Human Anatomy and Embryology, Faculty of Medicine, Assiut University. | ||
| 2Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University. | ||
| 3Department of Histology, Faculty of Medicine, Kafrelsheikh University. | ||
| 4Department of Human Anatomy and Embryology, Faculty of Medicine, Kafrelsheikh University. | ||
| Abstract | ||
| ABSTRACT Background: The bone marrow has been considered as a main source for isolation of multipotent mesenchymal stem cells (MSCs). They are still the most frequently investigated cell type and often identified as the gold standard. However, a similar cell population has been also isolated from other tissues such as adipose tissue. Unlike bone marrow, the adipose tissue is abundantly accessible source of stem cell that can give a good yield in culture. Aim of the work: Was isolation of the rat bone marrow MSCs (BM-MSCs) and adipose tissue MSCs (AD-MSCs) and assessing their growth kinetics. Material and Methods: The rat bone marrow and adipose tissue were isolated from 10 male adult albino rats and cultured and expanded through 6 passages. BM-MSCs and AD-MSCs biological characteristics evaluated for cell therapy (morphology, flow cytometric analysis, colony-forming unit-fibroblast assay, proliferation capacity at passages 2, 4 and 6, population doubling time (PDT) and cell growth curves). Results: BM-MSCs and AD-MSCs attached to the culture flask and displayed spindle-shaped morphology, more evident in AD-MSCs. Proliferation rate of AD-MSCs in the analyzed passages was more than BM-MSCs. The increase in the PDT of both types of MSCs occurred with the increase in the number of passages. Conclusion: The rat AD- MSCs have growth kinetic advantages in the proliferative capacity, colony-forming unite fibroblast and population doubling time more than that of BM-MSCs. these advantages should be considered when choosing a stem cell source for specific clinical application. | ||
| Keywords | ||
| growth kinetics; Mesenchymal Stem Cells; Bone marrow; adipose tissue; adult male albino rat | ||
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