GC-MS and LC-MS Analysis Unveil the Multifaceted Bioactivity of Eremina desertorum Snail Mucus: Antimicrobial, Antioxidant, Wound Regeneration, and Anticancer Efficacy Augmented by Molecular Docking Insights | ||
Bulletin of Pharmaceutical Sciences Assiut University | ||
Articles in Press, Accepted Manuscript, Available Online from 24 June 2025 | ||
Document Type: Original Article | ||
DOI: 10.21608/bfsa.2025.367074.2471 | ||
Authors | ||
Sheriene E. Ali* 1; Amina M. Ibrahim2; Shimaa Attia Atta3; Marwa A. Hamada4; Irene Sameh Gamil5; Heba El-Sayed4; Mostafa Y. Morad5 | ||
1Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt. | ||
2Medical Malacology Department, Theodor Bilharz Research Institute, Giza, Egypt | ||
3Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt | ||
4Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo, Egypt | ||
5Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt | ||
Abstract | ||
This study investigated the biological properties of Eobania desertorum snail mucus, focusing on its antimicrobial, antioxidant, wound healing, and antitumor activities. Gas chromatography-mass spectrometry (GC-MS) analysis identified key compounds, including triphenylmethane, palmitic acid, and pentadecanoic acid. In contrast, liquid chromatography-mass spectrometry (LC/MS) detected metabolites such as betaine, thiamine, leucine, and tryptophan. The mucus exhibited low antibacterial but moderate antifungal activity. It showed strong antioxidant potential, effectively scavenging DPPH free radicals (IC50 = 8.88 µg/mL). Wound healing was significantly enhanced in treated mice and in vitro scratch assays. The mucus also displayed potent anticancer effects, significantly inhibiting HepG-2 liver cancer (IC50 = 3.6 mg/mL) and CaCo-2 colorectal cancer (IC50 = 0.2 µg/mL) cell proliferation. Molecular docking revealed strong interactions of hexadecanoic acid with cancer and microbial receptors. These important findings suggest that E. desertorum mucus is a promising natural resource for biomedical applications, including wound healing, antioxidants, and innovative cancer therapy. | ||
Keywords | ||
Eremina desertorum; antimicrobial; wound healing; Caco-2cell lines; HepG-2 cell line | ||
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