GC-MS and LC-MS Analysis Unveil the Multifaceted Bioactivity of Eremina desertorum Snail Mucus: Antimicrobial, Antioxidant, Wound Regeneration, and Anticancer Efficacy Augmented by Molecular Docking Insights | ||||
Bulletin of Pharmaceutical Sciences Assiut University | ||||
Articles in Press, Accepted Manuscript, Available Online from 24 June 2025 | ||||
Document Type: Original Article | ||||
DOI: 10.21608/bfsa.2025.367074.2471 | ||||
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Authors | ||||
Sheriene E. Ali ![]() ![]() | ||||
1Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt. | ||||
2Medical Malacology Department, Theodor Bilharz Research Institute, Giza, Egypt | ||||
3Immunology Department, Theodor Bilharz Research Institute, Giza, Egypt | ||||
4Botany and Microbiology Department, Faculty of Science, Helwan University, Cairo, Egypt | ||||
5Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt | ||||
Abstract | ||||
This study investigated the biological properties of Eobania desertorum snail mucus, focusing on its antimicrobial, antioxidant, wound healing, and antitumor activities. Gas chromatography-mass spectrometry (GC-MS) analysis identified key compounds, including triphenylmethane, palmitic acid, and pentadecanoic acid. In contrast, liquid chromatography-mass spectrometry (LC/MS) detected metabolites such as betaine, thiamine, leucine, and tryptophan. The mucus exhibited low antibacterial but moderate antifungal activity. It showed strong antioxidant potential, effectively scavenging DPPH free radicals (IC50 = 8.88 µg/mL). Wound healing was significantly enhanced in treated mice and in vitro scratch assays. The mucus also displayed potent anticancer effects, significantly inhibiting HepG-2 liver cancer (IC50 = 3.6 mg/mL) and CaCo-2 colorectal cancer (IC50 = 0.2 µg/mL) cell proliferation. Molecular docking revealed strong interactions of hexadecanoic acid with cancer and microbial receptors. These important findings suggest that E. desertorum mucus is a promising natural resource for biomedical applications, including wound healing, antioxidants, and innovative cancer therapy. | ||||
Keywords | ||||
Eremina desertorum; antimicrobial; wound healing; Caco-2cell lines; HepG-2 cell line | ||||
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