Alterations In Inflammatory Biomarkers Among Egyptian Covid-19 Patients | ||||
| Egyptian Journal of Chemistry | ||||
| Articles in Press, Accepted Manuscript, Available Online from 25 June 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2025.369599.11478 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Hala Mohamed Ismail1; Wafaa Gh. Shousha2; olfat gamil shaker3; Shimaa Shawki Ramadan 4; Marian V. Philips5; Sara M. Abdo  6
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| 1Biochemistry Faculty of science Helwan University | ||||
| 2Chemistry Department, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt. | ||||
| 3professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo | ||||
| 4Helwan University, EGYPT | ||||
| 5Department of internal medicine, Faculty of medicine, Cairo university, Giza , Egypt . | ||||
| 6Faculty of Science Helwan University | ||||
| Abstract | ||||
| COVID-19 is a highly contagious disease with pathogenic characteristics, similar to pneumonia. It is caused by the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), which led to the recent global pandemic. Persistent inflammation in COVID-19 patients is associated with Toll-Like Receptor-4 (TLR4) signaling. Host microRNAs can alter cytokine storm linked to SARS-CoV-2 infection and have been proposed as biomarkers for COVID-19 disease. This study aimed to investigate the expression levels of miR-182 and TLR4 along with their correlation with laboratory biomarkers and clinical features in COVID-19 patients. A total of 100 participants were included, comprising 70 COVID-19 patients and 30 healthy controls. The expression levels of miR-182 and TLR4 were determined using qRT-PCR. Significant differences were observed in laboratory biomarkers, including RBCs, Hemoglobin, Hematocrit (%), MCHC , PLTs, WBCs, INR, basophile , eosinophil, albumin, bilirubin ,LDH , ALT, AST, PT, creatinine, potassium (K) , random blood sugar , CRP, D-Dimer, RDW (%) and partial pressure of oxygen (pO2), and Bicarbonate (HCO3) between COVID-19 patients and controls. Furthermore, miR-182 expression level was significantly upregulated, whereas TLR4 gene expression level was significantly downregulated in COVID-19 patients compared to controls. Besides, Receiver Operating Characteristic (ROC) curve analysis revealed diagnostic performance for miR-182 and TLR4, with cutoff values of 3.2 (AUC =0.986 , 98.57 % sensitivity, 99.8% specificity) and 0.67 (AUC= 0.953 ,90 % sensitivity, 99.99 % specificity), respectively ( p<0.0001). Based on these results, miR-182 and TLR4 may serve as potential diagnostic biomarkers for COVID-19. | ||||
| Keywords | ||||
| miR-182; Toll Like Receptor-4 (TLR4); diagnostic biomarkers; COVID-19; SARS-CoV-2; inflammation | ||||
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