Molecular Characterization and Phylogenetic Analysis of Nematodes of Phataginus tricuspis (Pholidonta: Manis) (Weber, 1904) and Thryonomys swinderianus, Temminck 1827 (Rodentia: Thryonomyidae)
Ayodele, E., Adewumi, O., Taiwo, A., Akinsanya, B., Fadipe, T., Abdul, N. (2025). Molecular Characterization and Phylogenetic Analysis of Nematodes of Phataginus tricuspis (Pholidonta: Manis) (Weber, 1904) and Thryonomys swinderianus, Temminck 1827 (Rodentia: Thryonomyidae). EKB Journal Management System, 17(1), 189-199. doi: 10.21608/eajbsc.2025.435583
Elizabeth T. Ayodele; Olusoji O. Adewumi; Adubi Taiwo; Bamidele Akinsanya; Temitope O. Fadipe; Nafisat O. Abdul. "Molecular Characterization and Phylogenetic Analysis of Nematodes of Phataginus tricuspis (Pholidonta: Manis) (Weber, 1904) and Thryonomys swinderianus, Temminck 1827 (Rodentia: Thryonomyidae)". EKB Journal Management System, 17, 1, 2025, 189-199. doi: 10.21608/eajbsc.2025.435583
Ayodele, E., Adewumi, O., Taiwo, A., Akinsanya, B., Fadipe, T., Abdul, N. (2025). 'Molecular Characterization and Phylogenetic Analysis of Nematodes of Phataginus tricuspis (Pholidonta: Manis) (Weber, 1904) and Thryonomys swinderianus, Temminck 1827 (Rodentia: Thryonomyidae)', EKB Journal Management System, 17(1), pp. 189-199. doi: 10.21608/eajbsc.2025.435583
Ayodele, E., Adewumi, O., Taiwo, A., Akinsanya, B., Fadipe, T., Abdul, N. Molecular Characterization and Phylogenetic Analysis of Nematodes of Phataginus tricuspis (Pholidonta: Manis) (Weber, 1904) and Thryonomys swinderianus, Temminck 1827 (Rodentia: Thryonomyidae). EKB Journal Management System, 2025; 17(1): 189-199. doi: 10.21608/eajbsc.2025.435583

Molecular Characterization and Phylogenetic Analysis of Nematodes of Phataginus tricuspis (Pholidonta: Manis) (Weber, 1904) and Thryonomys swinderianus, Temminck 1827 (Rodentia: Thryonomyidae)

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Volume 17, Issue 1, June 2025, Page 189-199  XML PDF (725.13 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2025.435583
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Authors
Elizabeth T. Ayodele1; Olusoji O. Adewumi2; Adubi Taiwo1; Bamidele Akinsanya1; Temitope O. Fadipe3; Nafisat O. Abdul4
1Parasitology and Bioinformatics division, Department of Zoology, University of Lagos, Lagos, Nigeria.
2Distance Learning Institute, University of Lagos, Akoka, Yaba, Lagos, Nigeria.
3Molecular Biology Division, Federal Institute of Industrial Research, Oshodi, Lagos, Nigeria.
4Department of Plant Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun state. Nigeria.
Abstract
This study conducted molecular characterization of nematode parasites in three wild mammals—Philantomba maxwelli, Phataginus tricuspis, and Thryonomys swinderianus—collected from bushmeat markets in Lagos and Oyo states, Nigeria. Utilizing primers targeting the 28S rRNA (LSU391/501) and 18S rRNA/ITS regions (VrainF/AB28), nematodes from P. tricuspis and T. swinderianus were successfully amplified, while those from P. maxwelli showed poor amplification. Sequencing and phylogenetic analysis revealed Setaria digitata in P. tricuspis (99.37% homology with Japanese isolates) and Chabertia ovina in T. swinderianus (99.77% homology with Canadian isolates). The overall parasite prevalence was 23.2%, with P. maxwelli exhibiting the highest infection rate (41.7%), followed by P. tricuspis (27.8%) and T. swinderianus (14.5%). Nematodes dominated infections (86.8%), with co-infections of cestodes observed in P. tricuspis. Phylogenetic trees constructed using neighbor-joining methods and p-distance algorithms strongly supported the cladal grouping of S. digitata with reference sequences from Japan and Thailand, while C. ovina clustered with isolates from Canada and China.
Keywords
Chabertia ovina; nematodes; Setaria digitata; wild mammals; molecular characterization
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