Isolation, identification and genotyping of Mycobacterium tuberculosis isolated from sputum samples of Iraqi TB patients | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 26 June 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.383035.2883 | ||
| Authors | ||
| Teeba Alaa Hussein* ; Khetam Habeeb Rasool* | ||
| Department of Biology, College of Science, Mustansiriyah University, Iraq | ||
| Abstract | ||
| Background: The use of molecular tests such as LPA to detect mutations in specific genes responsible for the resistance to anti-TB drugs is more efficient, effective, and reduces the time required to diagnose cases from weeks to hours. Aim: Isolation and identification of Mycobacterium tuberculosis isolated from the sputum samples. Genotyping of Drug-Resistant Mycobacterium tuberculosis based on the pattern of mutations in rpoB, inhA, katG, and gyrA genes by LPA. Methods: 72 TB patients (out of 438), suspected TB, and under-treated TB cases were enrolled in this study. Sputum samples were examined by conventional and molecular methods. Results: The results of the DST of 72 isolates shown that the resistance to: RIF were 27 (37.50%), INH were 19 (26.38%), ETH were 14 (19.44%), FLQ (LEVO and MOXI) were 13 (18.05%); While the resistance to AMI was not detected (0.0%). The molecular identification by LPA of mutations in rpoB, inhA, katG, gyrA, gyrB, eis, and rrs genes were detected in, 27 (37.50%), 14 (19.44%), 5 (6.94%), 13 (18.05%), 0 (0.0%), 0 (0.0%), and 0 (0.0%) isolates, respectively. The mutant type (+ MUT3) of rpoB gene where the most common resist mutation which appear in 20 (27.77%) isolates. The most common Genotype was Genotype (G5), which involved the mutation of the rpoB gene with the mutation of the inhA gene (-WT8, +MUT3/ -WT1,+MUT1) respectively. Conclusions: The molecular method using Line Probe Assay was a very useful method for detecting resistance mutation and Genotyping of DR-MTB isolates. | ||
| Keywords | ||
| Mycobacterium tuberculosis; Line Probe Assay (LPA); DR-MTB Genotype | ||
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