Microbial Strategies for Mycotoxin Reduction: Endophytic Fungi and Yeast in Aflatoxin B1 and Citrinin Biocontrol
Abd-Ellah, R., Ismaiel, A., sayed, M., Abd El-Rahman, T. (2025). Microbial Strategies for Mycotoxin Reduction: Endophytic Fungi and Yeast in Aflatoxin B1 and Citrinin Biocontrol. EKB Journal Management System, 17(1), 217-238. doi: 10.21608/eajbsg.2025.435770
Rana M. A. Abd-Ellah; Ahmed A. Ismaiel; Mohsen A. sayed; Tahany M. A. Abd El-Rahman. "Microbial Strategies for Mycotoxin Reduction: Endophytic Fungi and Yeast in Aflatoxin B1 and Citrinin Biocontrol". EKB Journal Management System, 17, 1, 2025, 217-238. doi: 10.21608/eajbsg.2025.435770
Abd-Ellah, R., Ismaiel, A., sayed, M., Abd El-Rahman, T. (2025). 'Microbial Strategies for Mycotoxin Reduction: Endophytic Fungi and Yeast in Aflatoxin B1 and Citrinin Biocontrol', EKB Journal Management System, 17(1), pp. 217-238. doi: 10.21608/eajbsg.2025.435770
Abd-Ellah, R., Ismaiel, A., sayed, M., Abd El-Rahman, T. Microbial Strategies for Mycotoxin Reduction: Endophytic Fungi and Yeast in Aflatoxin B1 and Citrinin Biocontrol. EKB Journal Management System, 2025; 17(1): 217-238. doi: 10.21608/eajbsg.2025.435770

Microbial Strategies for Mycotoxin Reduction: Endophytic Fungi and Yeast in Aflatoxin B1 and Citrinin Biocontrol

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Volume 17, Issue 1, June 2025, Pages 217-238    PDF (1.03 M)
Document Type: Original Article
DOI: 10.21608/eajbsg.2025.435770
Authors
Rana M. A. Abd-Ellah1; Ahmed A. Ismaiel2; Mohsen A. sayed1; Tahany M. A. Abd El-Rahman1
1Department of Botany and Microbiology, Faculty of Science, Cairo University,Egypt.
2Department of Botany and Microbiology, Faculty of Science, Zagazig University,Egypt.
Abstract
The ubiquitous threat of Aflatoxin B1 (AFB1) and Citrinin (CIT), potent mycotoxins derived from Aspergillus and Penicillium species, necessitates innovative strategies for food safety. This study aimed to assess fungal contamination in commercially available, slightly damaged canned food products from Egyptian markets. Five Aspergillus species were isolated and tested for their AFB1 and CIT production capabilities. The study then investigated the potential of endophytic fungi isolated from Solanum nigrum and Urtica dioica to mitigate these mycotoxins via their metabolites and binding activities. Alternaria tenuissima (accession number PQ517551) emerged as the most effective endophytic fungal species for mycotoxin reduction; its purified extract (band 2) achieved a 90.17% reduction in CIT and a 21.09% reduction in AFB1. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of this extract revealed 12 bioactive metabolites, including five fatty acids with known anti-mycotoxigenic properties. Concurrently, Meyerozyma guilliermondii (accession number PV660158), the second most effective endophytic isolate, exhibited potent AFB1 binding, achieving reduction rates ranging from 48.56% to 87.42% under influence of cell viability, incubation period and pH of the medium. These findings highlight the potential of specific endophytic fungal strains as effective strategies for mitigating AFB1 and CIT contamination.
Keywords
Aflatoxin B1 (AFB1); Citrinin (CIT); Endophytic fungi; Bioactive metabolites; Binding properties
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