Antioxidant activity of purified amylase extracted from clinical and environmental isolates of Bacillus licheniformis | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 27 June 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.392274.2863 | ||||
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| Authors | ||||
Salam M Khafif 1; Sawsan Hassan Authman 1; Afrah Fahad Abdulkareem  2
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| 1Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq | ||||
| 2Department of Microbiology, College of Science, Mustansiriyah University, Baghdad, Iraq | ||||
| Abstract | ||||
| Background: Microorganisms are one of the primary sources of α-amylase because it have a quick generation period and bacillus spp. consider one of the best producer so this study determined the ability of Bacillus licheniformis to produce amylase enzyme, this done by purification the enzyme and evaluate the antioxidant activity. Methods: Clinical and environmental isolates of B. licheniformis were examined for production extracellular amylase. The enzyme was purified by using 80% ammonium precipitation, dialysis, and chromatography together with ion exchange and gel filtration. Results: The results showed that the molecular weight of enzyme was about 57.5 KDa and this was definite by SDS-PAGE, and the activity of amylase recorded 47.85 U/ml at 37 ºC and pH7.5. By using DPPH assay. The antioxidant activity of the purified amylase was evaluated and the DPPH radical scavenging activity reached 44.4% at a concentration of 150 µg/ml. Conclusion: The results exhibited enzyme substantial antioxidant ability in a way that dose-reliant on, suggesting it’s playing dual role as antioxidant functional hydrolytic biomolecule. Also results revealed the possible benefit of amylase enzyme that purified from Bacillus licheniformis in applications like food industry and pharmaceutical products as a natural source for antioxidant. | ||||
| Keywords | ||||
| Amylase; Antioxidant; Bacillus licheniformis | ||||
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