UreC gene detection of Proteus mirabilis isolated and identified from patients with UTI | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 29 June 2025 PDF (771.44 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.373132.2671 | ||
| Authors | ||
| Mohammed Mahdi Jawad; Mohammed A Fayidh* ; Aisha Sulaiman Obaid | ||
| Department of Biology, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq | ||
| Abstract | ||
| Background: Proteus mirabilis is commonly associated with urinary tract infections (UTIs), and many patients exhibit antibiotic resistance to this bacterium and others. This study aimed to isolate and identify P. mirabilis in patients suffering from UTIs and to investigate the molecular identification of the UreC gene. Methods: A total of 135 clinical samples were collected from patients at Kirkuk General Hospital in Kirkuk City, northern Iraq, between September and December 2024. The samples were collected as midstream urine samples in sterile cups from both sexes across various age groups. Results: The findings indicated that, when urine samples from UTI patients were cultured on blood agar and MacConkey agar, 32 samples (23.7%) tested positive for P. mirabilis. In contrast, 103 samples (76.3%) exhibited negative results for P. mirabilis. The isolates demonstrated significant antibiotic resistance, with 81.3% resistant to Vancomycin, 78.1% to Clindamycin, and 75.0% to Ceftazidime. Additionally, complete resistance (100%) was observed to Ampicillin. However, P. mirabilis showed considerable sensitivity to Amikacin (93.8%) and Imipenem (84.4%), and it exhibited 100% sensitivity to Tobramycin. Genetically, out of the 32 isolates, 19 (59.4%) of the P. mirabilis isolates carried the UreC gene, which acts as a virulence factor. Conclusions: This study found that the UreC gene, which codes a large subunit responsible for regulating urease enzyme synthesis in P.mirabilis, is notable for its widespread presence across all species of Proteus. | ||
| Keywords | ||
| UTIs; P. mirabilis; UreC gene; Virulence factor; Antibiotic-resistant | ||
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