Phylogenetic analysis of some virulence factors related to MRSA among nosocomial infections in Al-Nasiriyah City, Southern Iraq. | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 29 June 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.393923.2892 | ||||
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| Authors | ||||
Alaa Ali chalab  ; Saad Salman Hamim 
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| Department of Pathological Analysis, College of Science, University of Thi-Qar, Iraq | ||||
| Abstract | ||||
| Background: Staphylococcus aureus (S. aureus) is divided into two groups: methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). According to the World Health Organization, MRSA infections are associated with noticeably increased rates of septic shock, post-infection hospital stays, ICU duration of stay, ICU mortality, and infection-attributable mortality. The present study aimed to shed light on some MRSA virulence factors in nosocomial infections in Thi-Qar Province, Southern Iraq. Methods: Total of 120 swab samples were enrolled in the present study collected from each patients at three hospitals between December 2024 and February 2025. Fifty S. aureus isolates were subjected to conventional Polymerase Chain Reaction (PCR) test utilizing femA and icaA genes as an virulence factors. Twenty S. aureus PCR products for both genes were selected and subjected to partial DNA sequencing to follow up their possible relationship via the phylogenetic tree that was constructed by MEGA-11 software among what was recorded globally in GenBank. Finally, five local clones were registered and gained an official accession numbers. Results: The results revealed that all S. aureus isolates (n=50) were positive for femA and icaA genes among nosocomial patients. Five selected partial sequenced clones were recorded in GenBank under the official accession numbers of LC867028, LC867029, LC866516, LC866517 and LC866518. The phylogenetic trees that was constructed software showed that there were different molecular relationships among the local S. aureus isolated from nosocomial patients with analogous ones around the world. Conclusions: Staphylococcus aureus, especially MRSA, represent a wide spread medical problem especially in hospitals. There is an urgent need for effective diagnostic and preventive procedures. | ||||
| Keywords | ||||
| Staphylococcus aureus; nosocomial infections; Gene sequencing; Virulence factors | ||||
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