Impact of Cumulus-Oocyte Complexes Morphological Grades on In Vitro Maturation and Biochemical Profiles in Dromedary Camels | ||
| Zagazig Veterinary Journal | ||
| Volume 53, Issue 2, June 2025, Pages 143-152 PDF (408.5 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zvjz.2025.338452.1271 | ||
| Authors | ||
| Mohamed Eleam1; Gamal Shawki1; Nasser Ghanem2; Omar Farid3; Beshoy S.F. Khalil2; Ayman Mesalam* 1 | ||
| 1Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt | ||
| 2Animal Production Department, Faculty of Agriculture, Cairo University, Giza, Egypt | ||
| 3National Organization for Drug Control and Research, Giza, Egypt | ||
| Abstract | ||
| The dromedary camel holds significant economic value, but its reproductive limitations pose challenges to fertility. Assisted reproductive technologies (ARTs), such as in vitro maturation (IVM) and embryo production, offer potential solutions to enhance camel fertility. This study aimed to assess the impact of morphological properties of cumulus-oocyte complexes (COCs) on maturation rates of dromedary camel oocytes and biochemical markers in the spent IVM medium. COCs were classified into good- and poor-quality based on morphological assessment followed by IVM for 30 hours then cumulus expansion, polar body extrusion, and mitochondrial activity were evaluated. Biochemical markers, including antioxidants (superoxide dismutase (SOD), catalyze (CAT), glutathione (GSH)), adenosine triphosphate (ATP), nitric oxide (NO), and malondialdehyde (MDA), were also measured the spent IVM medium. Results showed significant differences between good- and poor-quality COCs in cumulus expansion (94.8% vs. 52.0%, P < 0.0001), polar body release (47.0% vs. 22.5%, P = 0.0003), and mitochondrial intensity (2.45 vs. 1.51, P < 0.0001). Good-quality COCs had significantly higher SOD, CAT, GSH, and ATP levels, while poor-quality COCs exhibited increased NO and MDA levels in the spent IVM medium where morphologically different grades of COCs were cultured. These findings highlight the critical role of COCs quality in determining the maturation success in dromedary camels. | ||
| Keywords | ||
| Dromedary camels; Oocyte quality; In vitro maturation; Mitochondrial intensity; Antioxidants | ||
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