Assessment of the effects of mesenchymal stem cells-derived microvesicles on rheumatoid arthritic male rats
Mohammed, D., Ahmed, O., Soliman, A., Gaber, S. (2025). Assessment of the effects of mesenchymal stem cells-derived microvesicles on rheumatoid arthritic male rats. EKB Journal Management System, 6(2), 151-165. doi: 10.21608/ejmr.2023.219672.1412
Doaa R. Mohammed; Osama M. Ahmed; Ayman S. Soliman; Safy S. Gaber. "Assessment of the effects of mesenchymal stem cells-derived microvesicles on rheumatoid arthritic male rats". EKB Journal Management System, 6, 2, 2025, 151-165. doi: 10.21608/ejmr.2023.219672.1412
Mohammed, D., Ahmed, O., Soliman, A., Gaber, S. (2025). 'Assessment of the effects of mesenchymal stem cells-derived microvesicles on rheumatoid arthritic male rats', EKB Journal Management System, 6(2), pp. 151-165. doi: 10.21608/ejmr.2023.219672.1412
Mohammed, D., Ahmed, O., Soliman, A., Gaber, S. Assessment of the effects of mesenchymal stem cells-derived microvesicles on rheumatoid arthritic male rats. EKB Journal Management System, 2025; 6(2): 151-165. doi: 10.21608/ejmr.2023.219672.1412

Assessment of the effects of mesenchymal stem cells-derived microvesicles on rheumatoid arthritic male rats

Egyptian Journal of Medical Research
Volume 6, Issue 2, April 2025, Page 151-165  XML PDF (660.06 K)
Document Type: Original Article
DOI: 10.21608/ejmr.2023.219672.1412
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Authors
Doaa R. Mohammed email 1; Osama M. Ahmed2; Ayman S. Soliman1; Safy S. Gaber1
1Department of Medical Physiology, Faculty of Medicine, Beni- Suef University, Egypt.
2Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, P.O. 62521, Box Egypt.
Abstract
Background: Rheumatoid arthritis (RA) is a chronic, progressive, autoimmune disease caused by a malfunction of the immune system. The aim of this study was to examine the anti-arthritic effects of mesenchymal stem cell (MSC) derived microvesicles (MVs). Method: Thirty male rats were divided into 3 equal groups (control, RA, and RA treated with microvesicles groups). Complete Freund’s adjuvant (CFA) was used to establish RA in the animals by subcutaneous injection of 100 µL CFA/rat into plantar region of right hind leg in two consecutive days. RA rats were intravenously injected with MVs (100 μg protein/rat/week) into lateral tail vein of CFA-induced arthritic rats for 3 weeks. Results: CFA injection was deleterious to the ankle joints. CFA-induced arthritic rats showed high levels of serum pro-inflammatory mediators, such as rheumatoid factor (RF), tumour necrosis-alpha (TNF-α), and interleukin-1β (IL-1β). MVs treatment significantly lowered these pro-inflammatory mediators and increased serum level of anti-inflammatory cytokine, IL-10. Conclusion: MSC MVs exerted anti-arthritic and anti-inflammatory effects in CFA-induced arthritic rats.
Keywords
Rheumatoid arthritis; Mesenchymal stem cell; microvesicles
References

  1. Wasserman, A. (2018). Rheumatoid arthritis: common questions about diagnosis and management. American Family Physician, 97(7), 455-462.
  2. Kelmenson, L. B., Wagner, B. D., McNair, B. K., Frazer‐Abel, A., Demoruelle, M. K., Bergstedt, D. T., ... & Deane, K. D. (2020). Timing of elevations of autoantibody isotypes prior to diagnosis of rheumatoid arthritis. Arthritis & Rheumatology, 72(2), 251-261.
  3. Khanna N, Kumar A, Pawar SV. A Review on Rheumatoid Arthritis Interventions and Current Developments. Curr Drug Targets. 2021;22(4):463-483. doi: 10.2174/1389450121999201125200558. PMID: 33243118.
  4. Klareskog, L., Rönnelid, J., Saevarsdottir, S., Padyukov, L., & Alfredsson, L. (2020). The importance of differences; On environment and its interactions with genes and immunity in the causation of rheumatoid arthritis. Journal of Internal Medicine, 287(5), 514-533.
  5. Pas HI, Winters M, Haisma HJ, Koenis MJ, Tol JL, Moen MH. (2017) Stem cell injections in knee osteoarthritis: a systematic review of the literature. Br J Sports Med. 2017 Aug;51(15):1125-1133.
  6. Lv, X., Wang, L., Zou, X., & Huang, S. (2021). Umbilical cord mesenchymal stem cell therapy for regenerative treatment of rheumatoid arthritis: opportunities and challenges. Drug Design, Development and Therapy, 3927-3936.
  7. Zaborowski, M. P., Balaj, L., Breakefield, X. O., & Lai, C. P. (2015). Extracellular vesicles: composition, biological relevance, and methods of study. Bioscience, 65(8), 783-797.
  8. Liu, Q., Yu, Z., Yuan, S., Xie, W., Li, C., Hu, Z., ... & Li, Y. (2017). Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer. Oncotarget, 8(8), 13048.
  9. Mohammadi MR, Riazifar M, Pone EJ, Yeri A, Van Keuren-Jensen K, Lässer C, Lotvall J, Zhao W. Isolation and characterization of microvesicles from mesenchymal stem cells. Methods. 2020 May 1;177:50-57. doi: 10.1016/j.ymeth.2019.10.010. Epub 2019 Oct 25. PMID: 31669353; PMCID: PMC7182501.
  10. El-Gaphar, O. A. M. A., Abo-Youssef, A. M., & Abo-Saif, A. A. (2018). Effect of losartan in complete freund’s adjuvant–induced arthritis in rats. Iranian Journal of Pharmaceutical Research: IJPR, 17(4), 1420.
  11. Smolen, J. S., Aletaha, D., S., Breedveld, F. C., Burmester, G. R., Bykerk, V., Dougados, M., Emery, & McInnes, I. B. (2018). Therapies for bone R. Lancet, 30173-30178.
  12. Cosenza, S., Ruiz, M., Toupet, K., Jorgensen, C., & Noël, D. (2017). Mesenchymal stem cells derived exosomes and microparticles protect cartilage and bone from degradation in osteoarthritis. Scientific reports, 7(1), 16214.
  13. Nasuti, C., Bordoni, L., Fedeli, D., & Gabbianelli, R. (2019). Effect of Nigella sativa oil in a rat model of adjuvant-induced arthritis. Multidisciplinary Digital Publishing Institute Proceedings, 11(1), 16.
  14. Sameh, S., Fayez, A.M., Abdelaziz, M. and Abou El-ezz, D. "Amelioration of Autoimmunity and Inflammation by Zinc Oxide Nanoparticles in Experimental Rheumatoid Arthritis." (2021).
  15. Akiyama, M., & Kaneko, Y. (2022). Pathogenesis, clinical features, and treatment strategy for rheumatoid arthritis-associated interstitial lung disease. Autoimmunity Reviews, 21(5), 103056.
  16. Firestein GS, McInnes IB. (2017) Immunopathogenesis of Rheumatoid Arthritis. Immunity. 2017 Feb 21;46(2):183-196.
  17. Jeoung, B. R., Lee, K. D., Na, C. S., Kim, Y. E., Kim, B., & Kim, Y. R. (2013). Ganghwaljetongyeum, an anti-arthritic remedy, attenuates synoviocyte proliferation and reduces the production of proinflammatory mediators in macrophages: the therapeutic effect of GHJTY on rheumatoid arthritis. BMC complementary and alternative medicine, 13(1), 1-6.
  18. Ota, Y., Niiro, H., Ota, S. I., Ueki, N., Tsuzuki, H., Nakayama, T., ... & Akashi, K. (2016). Generation mechanism of RANKL+ effector memory B cells: relevance to the pathogenesis of rheumatoid arthritis. Arthritis research & therapy, 18(1), 1-10.
  19. Kyurkchiev D, Bochev I, Ivanova-Todorova E, Mourdjeva M, Oreshkova T, Belemezova K, Kyurkchiev S. (2014) Secretion of immunoregulatory cytokines by mesenchymal stem cells. World J Stem Cells. 2014 Nov 26;6(5):552-70.
  20. Conforti, A., Scarsella, M., Starc, N., Giorda, E., Biagini, S., Proia, A., ... & Bernardo, M. E. (2014). Microvescicles derived from mesenchymal stromal cells are not as effective as their cellular counterpart in the ability to modulate immune responses in vitro. Stem cells and development, 23(21), 2591-2599.
  21. Fu, H., Hu, D., Zhang, L., & Tang, P. (2018). Role of extracellular vesicles in rheumatoid arthritis. Molecular immunology, 93, 125-132.
  22. Mateen, S., Moin, S., Shahzad, S., & Khan, A. Q. (2017). Level of inflammatory cytokines in rheumatoid arthritis patients: Correlation with 25-hydroxy vitamin D and reactive oxygen species. PloS one, 12(6), e0178879.
  23. Ahmed, E. A., Ahmed, O. M., Fahim, H. I., Mahdi, E. A., Ali, T. M., Elesawy, B. H., & Ashour, M. B. (2021). Combinatory effects of bone marrow-derived mesenchymal stem cells and indomethacin on adjuvant-induced arthritis in Wistar rats: roles of IL-1β, IL-4, Nrf-2, and oxidative stress. Evidence-Based Complementary and Alternative Medicine, 2021.
  24. Wang, L., Pei, S., Han, L., Guo, B., Li, Y., Duan, R., ... & Jia, Y. (2018). Mesenchymal stem cell-derived exosomes reduce A1 astrocytes via downregulation of phosphorylated NFκB P65 subunit in spinal cord injury. Cellular Physiology and biochemistry, 50(4), 1535-1559.
  25. Xia, C., Dai, Z., Jin, Y., & Chen, P. (2021). Emerging antioxidant paradigm of mesenchymal stem cell-derived exosome therapy. Frontiers in Endocrinology, 1523.
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