Isolation and Characterization of Indole-3-butyric Acid-producing Azospirillum Bacteria from Grapevine Rhizosphere | ||
| Assiut Journal of Agricultural Sciences | ||
| Article 21, Volume 56, Issue 2, April 2025, Pages 312-320 PDF (545.52 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajas.2025.330221.1465 | ||
| Authors | ||
| Amira S. Youssef* ; Hashem M. Mohamed M. Mohamed; Mamdouh A. Eissa; Galal A. S. Elgharably | ||
| Department of Soils and Water, Faculty of Agriculture, Assiut University, Assiut, Egypt. | ||
| Abstract | ||
| Ten isolates of Azospirillum bacteria named from Azo-1 to Azo-10 have been isolated from the grapevine rhizosphere. Azospirillum isolates in Dobereiner medium with 0.01% tryptophan were evaluated for their ability to produce Indole-3-butyric acid (IBA). Indole butyric acid (IBA) was determined by colorimetric Salkowski reaction. As a result, of ten Azospirillum isolates collected from grapevine rhizosphere 3 isolates (30%) bio-synthesized IBA below 9 μg/mL, 5 isolates (50%) produced IBA from 9 to 17 μg/mL, and 2 isolates (20%) were recorded this value from 22 to 29 μg/mL. The results show a great variation among the tested Azospirillum isolates in production capacities of IBA (from 5 to 29 μg/mL). The results also show that the isolate Azo-4 produced the highest amounts of IBA (29 μg/mL). The highest five of Azospirillum isolates (Azo-1, Azo-4, Azo-6, Azo-8 and Azo-9), which are capable of producing IBA (22, 29, 15, 14 and 17 μg/ ml, respectively), are identified by the cell morphological, physiological and biochemical characteristics. Based on all the identification characteristics, select isolates were identified as Azospirillum spp. | ||
| Keywords | ||
| Azospirillum; Grapevine; Indole-3-butyric acid; Rhizosphere; Salkowski reagent | ||
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