Studies on Methicillin-Resistant Staphylococcus aureus (MRSA) Infection Resistance for Antibiotic Using Nanoparticles and Essential Oil | ||||
| Journal of Basic and Environmental Sciences | ||||
| Volume 12, Issue 3, July 2025, Page 97-106 PDF (737.64 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbes.2025.389805.1020 | ||||
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| Authors | ||||
Marwa Mohamed Amer  1; Samir Hamdy Abd El-Aziz2; Nadia Mohamed Elwan 3; Ibrahim Mohamed Abd El-Aziz4; Attia Ahmed Attia5
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| 1Department of Microbiology Organization Benha University Address Benha, Egypt | ||||
| 2Department of Microbiology Organization Benha University Address Benha, Egypt | ||||
| 3Department of Tropical, Faculty of Medicine, Tanta University | ||||
| 4R&D Department of Egyptian company for blood transfusion services (EGy BLOOD-VACSERA) | ||||
| 5Assistant Professor of Molecular biololgy and Microbiology, Department of Botany and Microbiology, Faculty of Science, Benha University, Benha, Egypt | ||||
| Abstract | ||||
| Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a strain of S. aureus that has developed resistance to nearly all β-lactam antibiotics, posing serious treatment challenges. Recent studies have shown that metal oxide nanoparticles, particularly copper oxide (CuO), possess strong antimicrobial properties against various pathogens, including S. aureus. Additionally, rosemary and its essential oil are commonly used in the food industry due to their pleasant flavor and potent antioxidant effects. Therefore, this study aims to improve the effectiveness of antibiotic treatments for MRSA infections through the use of copper oxide nanoparticles in combination with natural rosemary oil. Patients: This study was conducted on fifty blood samples collected from Patients infected with methicillin resistant Staphylococcus aureus (MRSA) were obtained from clinical specimens. Patient samples will be recruited from ICUs, Tanta University hospital, from October 2020 to May 2023. Methods: The samples were inoculated into blood agar and MacConkey agar, then incubated both aerobically at 37°c for 24hours. Results: various combinations to nanoparticles and essential oil showed an enhanced activity to different antibiotic at incubation for 24 h compared to antibiotics individually. Where, the combination of copper oxide 50% concentration with Rosemary essential oil 60% concentration (inhibition zone mean 15.14 mm) showed improved to inhibition zone for different antibiotics to MRSA strains. Conclusions: copper oxide nanoparticles have good effect for MRSA at high concentration. Also, zinc oxide nanoparticles showed good effect at low concentration but less than copper oxide nanoparticles . | ||||
| Keywords | ||||
| MRSA; Methicillin-resistant Staph aureus; Copper oxide nanoparticles; Rosemary essential oil; Antibiotic resistance | ||||
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