Evaluation of Methyl Transferase-like 3 (METTL3) expression in Pancreatic Adenocarcinoma: an immunohistochemical study

Abd Allah, Marwa S; Elbalshy, Abd Ellatif M; Abdallah, Shrook S; Abdelbary, Aya H; Elgendy, Eman I

doi:10.21608/bmfj.2025.363085.2333

	Evaluation of Methyl Transferase-like 3 (METTL3) expression in Pancreatic Adenocarcinoma: an immunohistochemical study
Benha Medical Journal
Articles in Press, Accepted Manuscript, Available Online from 04 July 2025 PDF (1.27 MB)
Document Type: Original Article
DOI: 10.21608/bmfj.2025.363085.2333
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Authors
Marwa S Abd Allah¹; Abd Ellatif M Elbalshy²; Shrook S Abdallah ³; Aya H Abdelbary⁴; Eman I Elgendy⁵
¹Department of Pathology, Faculty of Medicine-Benha University
²Department of pathology, Faculty of Medicine, Benha University
³(M.B.B.CH), Demonstrator of Pathology, Faculty of Medicine-Benha University
⁴Department of Pathology, National Liver Institute Menoufia University
⁵Department of Pathology, Faculty of Medicine- Benha University
Abstract
Background: Pancreatic carcinoma is the 12th most common cancer worldwide. Pancreatic cancer is one of the leading causes of cancer death worldwide. Aim: Evaluation Of the role of Methyltransferase like 3 (METTL3) expression by immunohistochemistry in pancreatic ductal adenocarcinoma and its relation with clinicopathological data. Material and methods: This retrospective study analyzed 50 cases of pancreatic ductal adenocarcinoma (PDAC) and 15 normal pancreatic tissue samples from Egyptian patients, collected between 2019 and 2023 using formalin-fixed paraffin-embedded (FFPE) samples. METTL3 proteins was assessed by Immunohistochemistry in these cases Results: METTL3 expression was negative in 26% of cases, low in 36%, and high in 38% of PDAC cases. A significant positive relationship was found between METTL3 expression and histologic grade, primary tumor extent, regional lymph node involvement, and pathological Tumor-Node-Metastasis (TNM) staging (p<0.05). Additionally, METTL3 expression showed significant negative correlation with tumor infiltrating lymphocytes. Conclusion: The study reveals that METTL3, an N6-methyladenosine RNA methyltransferase, is upregulated in pancreatic ductal adenocarcinoma (PDAC) tissues, correlated with tumor size, grade, and advanced staging, suggesting potential therapeutic target.
Keywords
Pancreatic carcinoma; PDAC; METTL3


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