Production of Cellulases Via Solid State Fermentation by Different Fungal Isolates using Various Agricultural Wastes as a Substrate | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 16, Issue 6, June 2025, Page 99-104 PDF (539.62 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2025.397031.1115 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Nora M. I. Elbanawy; A. E. I. Selim; A. M. M. Elattaapy 
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| Microbiology Dept., Fac. of Agric., Mansoura Univ. | ||||
| Abstract | ||||
| Four local cellulolytic fungal isolates were chosen among 30 isolates according to CMC-ase production via SSF using clover straw as agricultural waste. Isolates were identified as Trichoderma asperellum, Aspergillus flavus, Aspergillus flavipes, Aspergillus terreus. Different agricultural wastes were used for CMC-ase production by the isolates and the combination of clover straw and wheat brane (1:1) proved to be the best for CMC-ase production by the four isolates, while low values of CMC-ase were noticed by all fungal isolates by using the other agricultural wastes. Molasses proved to be the best additional carbon source for CMC-ase production by all tested fungi, also cellulose and carboxymethyl cellulose enhanced CMC-ase production. All addition nitrogen sources improved CMC-ase production, yeast extract and ammonium sulphate were the best nitrogen sources for CMC-ase production by Aspergillus flavus and Trichoderma asperellum, while ammonium chloride proved to be the best nitrogen source for CMC-ase production by Aspergillus flavipes and Aspergillus terreus. Trichoderma asperellum is in the first order in CMC-ase production followed by Aspergillus flavus, Aspergillus flavipes and Aspergillus terreus. The Three isolates of Aspergillus flavus, Aspergillus terreus and Aspergillus flavipes proved to be good producers for FP-ase, while few amount of FP-ase is produced by Trichoderma asperellum. | ||||
| Keywords | ||||
| Agricultural wastes; Cellulases; CMC-ase; Solid state fermentation | ||||
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