Potent anti-inflammatory activity of African daisy rich in polyphenols and quinic acid derivatives on carrageenan-induced rat paw edema | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Articles in Press, Accepted Manuscript, Available Online from 06 July 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2025.365834.2467 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ahmed Zaher  1; Youstina A. Malak1, 2; Khaled M. Mohamed3; Ahmed Mohamed Ahmed Abdelmawla1; DeiaaEldeen Elsayed Abouzed4
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| 1Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt | ||||
| 2Department of Pharmacognosy, Faculty of Pharmacy, South Valley University, Qena, Egypt | ||||
| 3Department of Pharmacognosy, Faculty of Pharmacy, Fayoum University, 63514 Fayoum, Egypt | ||||
| 4Department of Pharmacology and Toxicology, Faculty of Pharmacy, Sohag University, Sohag, Egypt | ||||
| Abstract | ||||
| The current study aims to investigate the in vivo anti-inflammatory and in vitro antioxidant activities of the ethyl acetate fraction of Dimorphotheca ecklonis DC (Compositae), along with its phytochemical composition and safety in gastric tissue. Wistar rats were divided into seven groups to analyze histopathological changes in the paw and gastric tissues treated with extracts of D. ecklonis (African daisy), compared to both positive and negative controls. The thickness of the dermal layers across all groups was measured using ImageJ software. High-Performance Liquid Chromatography (HPLC) analysis and metabolite isolation techniques were employed to identify the phytochemical constituents of the ethyl acetate fraction. Additionally, in vitro antioxidant activity and antihistamine release were evaluated using DPPH and MTT assays. The ethyl acetate fraction of African daisy demonstrated significant anti-inflammatory activity comparable to that of indomethacin. No significant pathological changes were observed in the gastric tissues of the rats treated with D. ecklonis ethyl acetate extract. Thirteen polyphenols of various classes were detected in the ethyl acetate fraction, including kaempferol, luteolin, apigenin, chlorogenic acid, and 4-caffeoyl-5-feruloyl quinic acid methyl ester. The high phenolic and flavonoid content contributed to its potent antioxidant, antihistamine, and anti-inflammatory activities. Our study concluded that the ethyl acetate fraction of D. ecklonis exhibits significant anti-inflammatory, antioxidant, and antihistamine activities in both in vivo and in vitro assays. These potent effects are attributed to the synergistic interactions of phenolics and flavonoids, such as kaempferol, luteolin, apigenin, chlorogenic acid, and 4-caffeoyl-5-feruloyl quinic acid methyl ester. | ||||
| Keywords | ||||
| Anti-inflammatory; Dimorphotheca. ecklonis; Flavonoids; Quinic acid derivatives; Carrageenan induced paw edema | ||||
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