Prevalence of adherence factors and vancomycin resistant genes among Enterococcus faecalis isolated from women with urinary tract infection | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 06 July 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.395078.2908 | ||||
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| Authors | ||||
Bayar Ali Hussein  ; Ali Jaffar Saleem
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| Department of Biology, College of Education for Pure Science, University of Diyala, Iraq | ||||
| Abstract | ||||
| Background: Enterococcus faecalis is a facultative anaerobic gram-positive coccus, a saprophyte of the human gastrointestinal tract, and acts as an opportunistic pathogen that can cause a range of nosocomial infections, including urinary tract infections (UTIs). Aims : The current study was conducted for detection of the adherence genes and vancomycin-resistant genes in local Enterococcus faecalis isolated from women with UTIs. E. faecalis is thought to be one of the most significant causes of this infection recently in several hospitals in the province of Diyala, Iraq. Methods: One hundred and thirty-four urine samples were collected from actual cases of urinary tract infections during the period from September 2023 to January 2024 from women patients in Baqubah Teaching Hospital, Al-Batool Teaching Hospital for Maternity and Children, and the specialized consulting clinic in Baqubah City. Results: Twenty-four clinical isolates were diagnosed as E. faecalis; by percentage, it reached 17.9% based on microscopic examinations, morphological characteristics, and the molecular method of PCR, depending on the ddl gene. All isolates were tested for the agg, ace, esp, pil, vanA, and vanB genes; the PCR results showed that these genes were found in 70.8%, 8.3%, 8.3%, 8.3%, 16.7%, and 0% of the samples, respectively. Also, the results indicated that isolates that possess genes related to adhesion do not carry the vancomycin resistance gene, and the opposite is true. Conclusion: The high incidence of UTI caused by E. faecalis isolates that were vancomycin resistant may be due to reasons other than carrying vanA genes. The study also found that E. faecalis local isolates do not have the vanB gene. | ||||
| Keywords | ||||
| Enterococcus faecalisesp, ace, van A and van B genes; urinary tract infection | ||||
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